Comparative analysis of a BAC contig of porcine chromosome 13q31-q32 and human chromosome 3q21-q22.docVIP

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Comparative analysis of a BAC contig of porcine chromosome 13q31-q32 and human chromosome 3q21-q22.doc

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Comparative analysis of a BAC contig of porcine chromosome 13q31-q32 and human chromosome 3q21-q22

BMC Genomics BioMedCentral Research article Open Access Comparative analysis of a BAC contig of porcine chromosome 13q31-q32 and human chromosome 3q21-q22 Mario Van Poucke1, David Bourry1,2, Fran?ois Piumi3, Marc Mattheeuws1, Alex Van Zeveren1, Patrick Chardon3 and Luc J Peelman*1 Address: 1Department of Animal Genetics and Breeding, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, B-9820 Merelbeke, Belgium, 2Department of Organic Chemistry, Faculty of Sciences, Ghent University, Krijgslaan 281 S4, B-9000 Ghent, Belgium and 3Laboratoire de Radiobiologie et dEtude du Génome, UMR INRA-CEA, F-78352 Jouy en Josas cedex, France Email: Mario Van Poucke - Mario.VanPoucke@UGent.be; David Bourry - David.Bourry@UGent.be; Fran?ois Piumi - Francois.Piumi@jouy.inra.fr; Marc Mattheeuws - Marc.Mattheeuws@UGent.be; Alex Van Zeveren - Alex.VanZeveren@UGent.be; Patrick Chardon - Patrick.Chardon@jouy.inra.fr; Luc J Peelman* - Luc.Peelman@UGent.be * Corresponding author Published: 21 September 2005 Received: 10 August 2005 Accepted: 21 September 2005 BMC Genomics 2005, 6:133 doi:10.1186/1471-2164-6-133 This article is available from: /1471-2164/6/133 ? 2005 Van Poucke et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The gene(s) encoding the ETEC F4ab/ac receptors, involved in neonatal diarrhoea in pigs (a disease not yet described in humans), is located close to the TF locus on Sscr13. In order to reveal and characterize possible candidate genes encoding these receptors, a porcine physical map of the TF region is indispensable. Results: A contig of 33 BAC clones, covering approximately 1.35 Mb surrounding the TF locus on Sscr13q31-q32, was built by chromosome walking. A total of 22,552 bp fr