Comparative analysis of complete nucleotide sequence of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Thailand (US and EU genotypes).docVIP

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Comparative analysis of complete nucleotide sequence of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Thailand (US and EU genotypes).doc

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Comparative analysis of complete nucleotide sequence of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Thailand (US and EU genotypes)

Virology Journal BioMedCentral Research Open Access Comparative analysis of complete nucleotide sequence of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Thailand (US and EU genotypes) Alongkorn Amonsin*, Roongtham Kedkovid, Suphasawatt Puranaveja, Piya Wongyanin, Sanipa Suradhat and Roongroje Thanawongnuwech Address: Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Road, Patumwan, Bangkok 10330, Thailand Email: Alongkorn Amonsin* - alongkorn.A@chula.ac.th; Roongtham Kedkovid - roongtham_kk@; Suphasawatt Puranaveja - psuphasawatt@; Piya Wongyanin - p_puns@; Sanipa Suradhat - suradhat.s@chula.ac.th; Roongroje Thanawongnuwech - roongroje.t@chula.ac.th * Corresponding author Published: 16 September 2009 Received: 10 August 2009 Accepted: 16 September 2009 Virology Journal 2009, 6:143 doi:10.1186/1743-422X-6-143 This article is available from: /content/6/1/143 ? 2009 Amonsin et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Porcine reproductive and respiratory syndrome virus (PRRSV) is a causative agent of Porcine Reproductive and Respiratory Syndrome (PRRS). In this study, the complete nucleotide sequences of the selected two Thai PRRSV isolates, EU (01CB1) and US (01NP1) genotypes were determined since both isolates are the Thai prototypes. Results: 01CB1 and 01NP1 contain 14,943 and 15,412 nucleotides, respectively. The viruses compose 2 untranslated regions (5 UTR and 3 UTR) and 8 open reading frames (ORFs) designated as ORF1a, ORF1b and ORF2-7. Phylogenetic analysis of full length of the viruses also showed that the 01CB1 and 01NP1 were grouped into the EU and US genotype, respectively. In order to determine the genetic