Construction and characterization of a genomic BAC library for the Mus m. musculus mouse subspecies (PWDPh inbred strain).docVIP

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Construction and characterization of a genomic BAC library for the Mus m. musculus mouse subspecies (PWDPh inbred strain).doc

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Construction and characterization of a genomic BAC library for the Mus m. musculus mouse subspecies (PWDPh inbred strain)

BMC Genomics BioMedCentral Research article Open Access Construction and characterization of a genomic BAC library for the Mus m. musculus mouse subspecies (PWD/Ph inbred strain) Petr Jansa, Petr Divina and Ji?í Forejt* Address: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic and Center for Applied Genomics, Vídeňská 1083, CZ-142 20, Prague 4, Czech Republic Email: Petr Jansa - pjansa@biomed.cas.cz; Petr Divina - divina@biomed.cas.cz; Ji?í Forejt* - jforejt@biomed.cas.cz * Corresponding author Published: 16 November 2005 Received: 16 August 2005 Accepted: 16 November 2005 BMC Genomics 2005, 6:161 doi:10.1186/1471-2164-6-161 This article is available from: /1471-2164/6/161 ? 2005 Jansa et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The genome of classical laboratory strains of mice is an artificial mosaic of genomes originated from several mouse subspecies with predominant representation (90%) of the Mus m. domesticus component. Mice of another subspecies, East European/Asian Mus m. musculus, can interbreed with the classical laboratory strains to generate hybrids with unprecedented phenotypic and genotypic variations. To study these variations in depth we prepared the first genomic large insert BAC library from an inbred strain derived purely from the Mus m. musculus-subspecies. The library will be used to seek and characterize genomic sequences controlling specific monogenic and polygenic complex traits, including modifiers of dominant and recessive mutations. Results: A representative mouse genomic BAC library was derived from a female mouse of the PWD/Ph inbred strain of Mus m. musculus subspecies. The library consists of 144 768 pri