Anti-HIV Activity of Human Defensin 5 in Primary CD4+ T Cells under Serum-Deprived Conditions Is a Consequence of Defensin-Mediated Cytotoxicity.docVIP

下载本文档

3
0
约4.53万字
约 11页
2017-05-07 发布于上海
举报
版权申诉

Anti-HIV Activity of Human Defensin 5 in Primary CD4+ T Cells under Serum-Deprived Conditions Is a Consequence of Defensin-Mediated Cytotoxicity.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Anti-HIVActivityofHumanDefensin5inPrimaryCD4TCellsunderSerum-DeprivedConditionsIsaConsequenceofDefensin-MediatedCytotoxicity

Anti-HIVActivityofHumanDefensin5inPrimaryCD4+T CellsunderSerum-DeprivedConditionsIsa ConsequenceofDefensin-MediatedCytotoxicity JianDing1,CarleyTasker2,4,KimyataValere2,4,TiinaSihvonen1,DanteB.Descalzi-Montoya3,4, WuyuanLu5,TheresaL.Chang1,2* 1PublicHealthResearchInstitute,RutgersBiomedicalandHealthSciences-NewJerseyMedicalSchool,Newark,NewJersey,UnitedStatesofAmerica,2Departmentof MicrobiologyandMolecularGenetics,RutgersBiomedicalandHealthSciences-NewJerseyMedicalSchool,Newark,NewJersey,UnitedStatesofAmerica,3Departmentof Pathology and Laboratory Medicine, Rutgers Biomedical and Health Sciences-New Jersey Medical School, Newark, New Jersey, United States of America, 4Graduate SchoolofBiomedicalSciences,RutgersBiomedicalandHealthSciences-NewJerseyMedicalSchool,Newark,NewJersey,UnitedStatesofAmerica,5InstituteofHuman VirologyandDepartmentofBiochemistryandMolecularBiology,UniversityofMarylandSchoolofMedicine,Baltimore,Maryland,UnitedStatesofAmerica Abstract Background:Wehavepreviouslyshownthathumandefensin5(HD5)promotesHIVinfectivityinbothprimaryCD4+Tcells and HeLa cells expressing CD4 and CCR5. HD5 is induced in response to sexually transmitted infections (STIs) such as Chlamydia trachomatis and Neisseria gonorrhoeae, suggesting it plays a role in STI-mediated enhancement of HIV transmission.Incontrasttoourfindings,arecentstudyreportsthatHD5hasananti-HIVeffectinprimaryCD4+Tcellsunder serum-deprived conditions. To resolve these apparently contradictory observations, we investigated experimental parametersthatmightcontributetocontrastingeffectsofHD5. Results:Serum-deprivedcultureconditionswereassociatedwithanti-HIVactivity.IncontrasttothedependenceoftheHIV enhancingeffectonHD5structure,theanti-HIVactivityinserum-deprivedprimaryCD4+TcellswasindependentofHD5 structureasthelinearpeptide[Abu]HD5exhibitedsimilaranti-HIVactivity.Underserumdeprivedconditions,HD5blocked CD4-receptor-independent HIV-1 infection before or after viral entry. We found that HD5 and its linear f