硒化壳寡糖和硒化羧甲基壳聚糖中硒含量的测定.docVIP

Bioprocess 生物过程, 2011, 1, 1-4 /10.12677/bp.2011.11001 Published Online September 2011 (/journal/bp/) Determination of Selenium in Chitooligosaccharide- Selenium and Selenium Carboxymethyl-Chitosan# Wenying Quan , Xianling Qin , Peng Cao , Xiaohui Qi , Shengdi Fan , Liming Jin 1 1 2,3 1 1 1* 1 College of Life Science, Dalian Nationalities University, Dalian 2 College of Life Science, Jinan University, Guangzhou Yantai Entry-Exit Inspection and Quarantine Bureau, Yantai Email: jlm@ 3 Received: Sep. 12th, 2011; revised: Sep. 21st, 2011; accepted: Sep. 25th, 2011. Abstract: Sodium selenite and chitooligosaccharide, carboxymethyl-chitosan, respectively, were used to synthesize chitooligosaccharide-selenium and selenium carboxymethyl-chitosan in this experiment by chem- ical method. The contents of selenium were tested by o-phenylenediamine and diaminobenzidine spectro- photometric methods. The results showed the consistence of the two methods. The contents of selenium in chitooligosaccharide-selenium were 9.02 mg/g, 8.82 mg/g, respectively. The contents of selenium in selenium carboxymethyl-chitosan were 20.50 mg/g, 20.24 mg/g, respectively. Keywords: Chitooligosaccharide-Selenium; Selenium Carboxymethyl-Chitosan; O-Phenylenediamine Spectrophotometric; Diaminobenzidine Spectrophotometric 硒化壳寡糖和硒化羧甲基壳聚糖中硒含量的测定 # 全文颖 ，覃仙玲 ，曹 鹏 ，齐小辉 ，范圣第 ，金黎明 1* 1 1 2,3 1 1 1 大连民族学院，生命科学学院，大连 2 暨南大学生命科学学院，广州 烟台出入境检验检疫局，烟台 Email: jlm@ 3 收稿日期：2011年 9月 12日；修回日期：2011年 9月 21日；录用日期：2011年 9月 25日 摘 要：分别以亚硒酸钠与壳寡糖、羧甲基壳聚糖为原料，合成硒化壳寡糖和硒化羧甲基壳聚糖。分 别用邻苯二胺分光光度法和二氨基联苯胺分光光度法测定其中的硒含量，两种方法测定的结果较为一 致，测得硒化壳寡糖中硒含量分别为 9.02 mg/g、8.82 mg/g，测得硒化羧甲基壳聚糖中硒含量分别为 20.50 mg/g、20.24 mg/g。 关键词：硒化壳寡糖；硒化羧甲基壳聚糖；邻苯二胺分光光度法；二氨基联苯胺分光光度法 1. 引言 素吸收等方面有显著的功效。羧甲基壳聚糖 (carboxy- methyl-chitosan)是壳聚糖经化学改性得到的水溶性衍 生物，其溶解性好、络合金属离子能力强，可作为水