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Analysis of Several mRNA in Human Meningiomas英文文献资料
HindawiPublishingCorporation
MediatorsofIn?ammation
Volume2009,ArticleID689430,8pages
doi:10.1155/2009/689430
ResearchArticle
AnalysisofSeveralPLA2mRNAinHumanMeningiomas
YvesDenizot,
1
RafaelDeArmas,
3
2
KarineDurand, SandrineRobert, Jean-JacquesMoreau,
2 2 3
andFranc?oisLabrousse
2
Franc?oisCaire,
NicolasWeinbreck,
2
1
UMRCNRS6101,CentreNationaldelaRechercheScienti?que,Facult′edeM′edecine,
Universit′edeLimoges,2rueDr.Marcland,87025Limoges,France
2
3
Serviced’AnatomiePathologique,CHUDupuytren,87045Limoges,France
ServicedeNeurochirurgie,CHUDupuytren,87045Limoges,France
CorrespondenceshouldbeaddressedtoYvesDenizot,yves.denizot@unilim.fr
Received7September2009;Revised25November2009;Accepted21December2009
RecommendedbyMuzamilAhmad
InviewoftheimportantoncogenicactionofphospholipaseA2(PLA2)weinvestigatedPLA2transcriptsinhumanmeningiomas.
Real-timePCRwasusedtoinvestigatePLA2 transcriptsin26humanmeningiomatumors.ResultsindicatedthatthreeCa
dependenthighmolecularweightPLA2(PLA2-IVA,PLA2-IVB,PLA2-IVC),oneCa -independenthighmolecularweightPLA2
2+
-
2+
(PLA2-VI) and ?ve low molecular weight secreted forms of PLA2 (PLA2-IB, PLA2-IIA, PLA2-III, PLA2-V, and PLA2-XII) are
expressedwithPLA2-IVA,PLA2-IVB,PLA2-VI,andPLA2-XIIAasthemajorexpressedforms.PLA2-IIE,PLA2-IIF,PLA2-IVD,
andPLA2-XIIBarenot detected. Plasma (PLA2-VIIA)andintracellular (PLA2-VIIB)platelet-activating factoracetylhydrolase
transcriptsareexpressedinhumanmeningiomas.Howevernodi?erencewasfoundforPLA2transcriptamountsinrelationto
thetumorgrade,thesubtypeofmeningiomas,thepresenceofin?ammatoryin?ltratedcells,ofanassociatededema,mitosis,
braininvasion,vascularisationornecrosis.InconclusionnumerousgenesencodingmultiplesformsofPLA2 areexpressedin
meningiomaswheretheymightactonthephospholipidremodelingandonthelocaleicosanoidand/orcytokinenetworks.
Copyright?2009YvesDenizotetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense,
whichpermitsu
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