Analysis of Several mRNA in Human Meningiomas英文文献资料.docVIP

Analysis of Several mRNA in Human Meningiomas英文文献资料.doc

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Analysis of Several mRNA in Human Meningiomas英文文献资料

HindawiPublishingCorporation MediatorsofIn?ammation Volume2009,ArticleID689430,8pages doi:10.1155/2009/689430 ResearchArticle AnalysisofSeveralPLA2mRNAinHumanMeningiomas YvesDenizot, 1 RafaelDeArmas, 3 2 KarineDurand, SandrineRobert, Jean-JacquesMoreau, 2 2 3 andFranc?oisLabrousse 2 Franc?oisCaire, NicolasWeinbreck, 2 1 UMRCNRS6101,CentreNationaldelaRechercheScienti?que,Facult′edeM′edecine, Universit′edeLimoges,2rueDr.Marcland,87025Limoges,France 2 3 Serviced’AnatomiePathologique,CHUDupuytren,87045Limoges,France ServicedeNeurochirurgie,CHUDupuytren,87045Limoges,France CorrespondenceshouldbeaddressedtoYvesDenizot,yves.denizot@unilim.fr Received7September2009;Revised25November2009;Accepted21December2009 RecommendedbyMuzamilAhmad InviewoftheimportantoncogenicactionofphospholipaseA2(PLA2)weinvestigatedPLA2transcriptsinhumanmeningiomas. Real-timePCRwasusedtoinvestigatePLA2 transcriptsin26humanmeningiomatumors.ResultsindicatedthatthreeCa dependenthighmolecularweightPLA2(PLA2-IVA,PLA2-IVB,PLA2-IVC),oneCa -independenthighmolecularweightPLA2 2+ - 2+ (PLA2-VI) and ?ve low molecular weight secreted forms of PLA2 (PLA2-IB, PLA2-IIA, PLA2-III, PLA2-V, and PLA2-XII) are expressedwithPLA2-IVA,PLA2-IVB,PLA2-VI,andPLA2-XIIAasthemajorexpressedforms.PLA2-IIE,PLA2-IIF,PLA2-IVD, andPLA2-XIIBarenot detected. Plasma (PLA2-VIIA)andintracellular (PLA2-VIIB)platelet-activating factoracetylhydrolase transcriptsareexpressedinhumanmeningiomas.Howevernodi?erencewasfoundforPLA2transcriptamountsinrelationto thetumorgrade,thesubtypeofmeningiomas,thepresenceofin?ammatoryin?ltratedcells,ofanassociatededema,mitosis, braininvasion,vascularisationornecrosis.InconclusionnumerousgenesencodingmultiplesformsofPLA2 areexpressedin meningiomaswheretheymightactonthephospholipidremodelingandonthelocaleicosanoidand/orcytokinenetworks. Copyright?2009YvesDenizotetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, whichpermitsu

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