Analysis of the arginine biosynthetic gene cluster argCJBDFR of Corynebacterium crenatum英文文献资料.docVIP

J. Biomedical Science and Engineering, 2011, 4, 70-75 JBiSE doi:10.4236/jbise.2011.41009 Published Online January 2011 (http://www.SciRP.org/journal/jbise/). Analysis of the arginine biosynthetic gene cluster argCJBDFR of Corynebacterium crenatum Haitao Jiao , Yong Yuan , Yonghua Xiong , Xuelan Chen 1 2 2 1 1 2 College of life sciences, Jiangxi Normal University, Nanchang, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China. Email: yhxiongchen@163.com, xuelanchen14@ Received 8 October 2010; revised 28 October 2010; accepted 8 November 2010. ABSTRACT coded acetylornithinase catalyses the hydrolysis of N-acetylornithine into the arginine precursor ornithine and acetate; the other is called the “economic cyclic” pathway, in which acetylornithine is catalyzed into or- nithine and acetyl groups, and recycled with generation of acetylglutamate by argJ gene coded ornithine acetyl- transferase [2]. ArgJ has both acetylornithinase (coded by argE) function and N-acetylglutamate synthase (coded by argA) functions in the “cyclic” pathway. Lit- eratures showed that Enterobacteriaceae and Sulfolobus solfataricus [2,3] adopted the “linear” pathway in which the metabolic flow is controlled by arginine-mediated feedback inhibition of the first biosynthetic step； all the other prokaryotes, including Methanogenic archaea, Neisseria gonorrboese, members of the genus Bacillus and the eukaryotic microbes, use the “cyclic” pathway in which the metabolic flow was controlled by argin- ine-mediated feedback inhibition of the second biosyn- thetic step or by orthinine-mediated feedback inhibition of the fifth step [2]. Although the argJ gene by itself would thus be able to assure both the first and the fifth steps of arginine biosynthes