BacMam Virus Transduced Cardiomyoblasts Can Be Used for Myocardial Transplantation Using AP-PEG-A Microcapsules Molecular Cloning, Preparation, and In Vitro Analysis英文文献资料.docVIP

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BacMam Virus Transduced Cardiomyoblasts Can Be Used for Myocardial Transplantation Using AP-PEG-A Microcapsules Molecular Cloning, Preparation, and In Vitro Analysis英文文献资料.doc

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BacMam Virus Transduced Cardiomyoblasts Can Be Used for Myocardial Transplantation Using AP-PEG-A Microcapsules Molecular Cloning, Preparation, and In Vitro Analysis英文文献资料

HindawiPublishingCorporation JournalofBiomedicineandBiotechnology Volume2010,ArticleID858094,12pages doi:10.1155/2010/858094 ResearchArticle BacMamVirusTransducedCardiomyoblastsCanBeUsedfor MyocardialTransplantationUsingAP-PEG-AMicrocapsules: MolecularCloning,Preparation,andInVitroAnalysis ArghyaPaul, AfshanAfsarKhan, DominiqueShum-Tim, 1 1 2andSatyaPrakash 1,3 1 2 3 BiomedicalTechnologyandCellTherapyResearchLaboratory,DepartmentofBiomedicalEngineering,FacultyofMedicine, McGillUniversity,3775UniversityStreet,Montreal,QC,CanadaH3A2B4 DivisionsofCardiacSurgeryandSurgicalResearch,TheMontrealGeneralHospital,1650CedarAvenue,SuiteC9-169, Montreal,QC,CanadaH3G1A4 Arti?cialCellsandOrgansResearchCentre,FacultyofMedicine,McGillUniversity,Montreal,QC,CanadaH3G1Y6 CorrespondenceshouldbeaddressedtoSatyaPrakash,satya.prakash@mcgill.ca Received5June2010;Revised14September2010;Accepted10November2010 AcademicEditor:MohamedBoutjdir Copyright?2010ArghyaPauletal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. Thepotentialofgeneticallymodi?edcardiomyoblastsintreatingdamagedmyocardiumiswellknown.However,e?cientdelivery ofthesecellsisofmajorconcernduringtreatment.Thelimitingfactorsarethemassivecelldeaththatoccurssoonaftertheir intramyocardial transplantation into the beating heart. To address these problems, we generated recombinant baculoviruses (BacMam viruses) which e?ciently transduced cardiomyoblast cells under optimized conditions. These genetically modi?ed cellswerethenprotectedinanewpolymericmicrocapsuleusingpoly-ethylene-glycol(PEG),alginate,andpoly-L-lysine(PLL) polymersfore?cientdelivery.Resultsshowedthatmicrocapsulesmaintaincellviabilityandsupportcellproliferationforatleast 30days.Thecapsulesexhibitstrongimmunoprotectivepotentialandhavehighmechanicalandosmoticstabilitywithmorethan 70%intactcaps