Bacteriostatic Effects of Hyaluronan-Based Bioresorbable Membrane英文文献资料.docVIP

Surgical Science, 2011, 2, 431-436 doi:10.4236/ss.2011.29094 Published Online November 2011 (http://www.SciRP.org/journal/ss) Bacteriostatic Effects of Hyaluronan-Based Bioresorbable Membrane Keiichi Uchida, Kohei Otake, Mikihiro Inoue, Yuhki Koike, Kohei Matsushita, Koji Tanaka, Yasuhiro Inoue, Yasuhiko Mohri, Masato Kusunoki Department of Gastrointestinal and Pediatric Surgery, Graduate School of Medicine, Mie University, Tsu, Mie, Japan E-mail: ucchie@clin.medic.mie-u.ac.jp Received June 14, 2011; revised September 16, 2011; accepted October 31, 2011 Abstract Purpose: The purpose of this study is to determine the presence of bacteriostatic effects of hyaluronan-based bioresorbable membrane (HA/CMC) on selected major bacterial strains in digestive organs. Methods: We firstly evaluated the growth inhibition effect of HA/CMC for E. coli and S. aureus by determining the optical density (OD)650 in the incubation medium. At second, to determine the viable counts of bacteria, total adeno- sine triphosphate (ATP) were measured with five groups; several concentrations of HA/CMC and control. Results: OD curve gradually elevated and reached to plateau at 4 hours in E. coli. and 6 hours in S. aureus. After reaching plateau, the growth inhibition of both strains was statistically significantly correlated to the concentrations of HA/CMC. The ATP productions had statistically significant differences at 6 hours after incubation and inhibited in dose-dependence of a well-dissolved HA/CMC. Conclusion: HA/CMC may have dose-dependently bacteriostatic effects on S. aureus and E. coli. Keywords: HA/CMC, Bacteriostatic, Hyaluronan, Adhesion, Surgery 1. Introduction dels of inflammation and endotoxin-stimulation. Al- though our results showed that HA/CMC can be safely used even in contaminated conditions, further basal ex- periments about HA/CMC against infectious pathogens are needed. Clinical effectiveness and safety of hyaluronan-