Assessment of Antifungal Activity of Some Himalayan Foliose Lichens Against Plant Pathogenic Fungi英文文献资料.docVIP

American Journal of Plant Sciences, 2011, 2, 841-846 841 doi:10.4236/ajps.2011.26099 Published Online December 2011 (http://www.SciRP.org/journal/ajps) Assessment of Antifungal Activity of Some Himalayan Foliose Lichens against Plant Pathogenic Fungi Priti Tiwari 1,2 , Himanshu Rai 1 1* , Dalip Kumar Upreti , Suman Trivedi 2 , Preeti Shukla 1 1 Lichenology Laboratory, CSIR—National Botanical Research Institute, Lucknow, India; lah University, Bhopal, India. 2 Motilal Vigyan Mahavidyalaya, Barkatul- Email: upretidk@ * Received July 13 , 2011; revised October 7 , 2011; accepted October 21 , 2011. th th st ABSTRACT In vitro antifungal activity of the acetone , methanol and chloroform extracts of four lichen species viz , Bulbothirx set- schwanensis, Everniastrum nepalense, Heterodermia diademata, Parmelaria thomsonii were investigated against seven plant pathogenic fungi (Aspergillus flavus, A. fumigatus, Alternaria alternata, Fusarium oxysporum, F. solani, F. rose- um and Penicillium citrinum) with reference to commercially available synthetic antifungal drug Ketoconazole (positive control). Lichen secondary metabolites were extracted using Soxhlet extractor and were further recovered through gen- tle evaporation of solvents in rotary evaporator. Antifungal activity was analysed employing Bauer-Kirby disc diffu- sion assay. Acetone and methanol extracts of lichenized fungi were found more effective against tested plant pathogenic fungi. Principal component analysis concluded that though , Ketoconazole was effective against four of the tested plant pathogenic fungi, acetone and methanol extracts of lichens were comparatively more effective against some broad spectrum plant pathogenic fungi (Fusarium oxysporum, F. solani, F. roseum). Keywords: Acetone Extract, Antifungal Activity, Bauer-Kirby Disc Diffusion As