Application of an Impedimetric Technique for the Detection of Lytic Infection of Salmonella spp. by Specific Phages英文文献资料.docVIP

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Application of an Impedimetric Technique for the Detection of Lytic Infection of Salmonella spp. by Specific Phages英文文献资料.doc

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Application of an Impedimetric Technique for the Detection of Lytic Infection of Salmonella spp. by Specific Phages英文文献资料

HindawiPublishingCorporation InternationalJournalofMicrobiology Volume2009,ArticleID259456,6pages doi:10.1155/2009/259456 ResearchArticle ApplicationofanImpedimetricTechniquefortheDetectionof LyticInfectionofSalmonellaspp.bySpeci?cPhages LaraR.P.Amorim,JoanaG.L.Silva,PaulA.Gibbs,andPaulaC.Teixeira EscolaSuperiordeBiotecnologia,UniversidadeCat′olicaPortuguese,RuaDr.Ant′onioBernardinodeAlmeida, 4200-072Porto,Portugal CorrespondenceshouldbeaddressedtoPaulaC.Teixeira,paula@esb.ucp.pt Received13May2009;Accepted8September2009 RecommendedbyToddR.Callaway ThisstudywasperformedtoevaluatetheadaptionoftheimpedimetricmethodtodetectthelyticinfectionbySalmonella-speci?c bacteriophagesandtoprovideahigherselectivitytothisrapidmethodindetectingSalmonellaspp.byusingspeci?cagents.Three bacteriophagesandtwelvestrainsofSalmonellaspp.weretested.EachofthetwelvestrainswasusedseparatelytoinoculateTSB togetherwitheachoneofthephages.Theinoculumconcentrationwasbetween10 Fromthesampleanalysis,basedonconductance(G)measurements(37 C),theinfectioncouldbedetected,byobservationofboth and10 cfu/mL,atacell:phageratioof1:100. 6 7 ? detection-timedelayanddistinctcurvetrends.Themainconclusionswerethatkineticdetectionbyimpedancemicrobiologywith phagetypingconstitutesamethodofdeterminingwhetheratestmicroorganismissensitivetothebacteriophageandamethodto evaluatewhetheralyticbacteriophageispresentinasample,bya?ectingbacterialgrowthrate/metabolicchange. Copyright?2009LaraR.P.Amorimetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. 1.Introduction Forafoodprocessorproducingastandardproductunder verysimilarconditionseachday,acalibrationcurveforthat product can be established and used in quickly evaluating the microbial status, but only as long as the conditions of the food production, media, and temperature used in the instrument, and so forth, remain the same [5