Angiogenesis in Rat Uterine Scar after Introduction af Autological Mesenchymal Stem Cells of Bone Marrow Origin英文文献资料.docVIP

J. Biomedical Science and Engineering, 2011, 4, 164-172 JBiSE doi:10.4236/jbise.2011.43023 Published Online March 2011 (http://www.SciRP.org/journal/jbise/). Angiogenesis in rat uterine scar after introduction after autological mesenchymal stem cells of bone marrow origin Igor Maiborodin, Natalia Yakimova, Vera Matveeva, Andrey Shevela, Elena Maiborodina, Ekaterina Pekareva, Olesya Tkachuk Center of New Medical Technologies Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia. Email: imai@mail.ru Received 24 August 2010; revised 10 September 2011; accepted 13 September 2010. ABSTRACT with the potential to transdifferentiation into cells of different phenotypes [2]. The results of injecting of autologic mesenchymal stem cells of bone marrow origin (AMSCBMO), trans- fected by the GFP gene, into the scar of rat uterine horns were studied by methods of light microscopy. After the introduction of AMSCBMO into the formed scar on the right (2 months after the ligation) large groups of blood vessels with cellular elements inside were present; groups like that were not found in the opposite side. Studying unstained sections under re- flected ultraviolet light the sufficient bright lumines- cence in the endothelium and the external membrane of scar vessels was found in uterine horn only on the side of introduction of AMSCBMO. It was concluded that after the introduction of AMSCBMO into the scar tissue they form blood vessels by differentiation into endotheliocytes and pericytes. GFP gene expres- sion not only in endothelium of vessels, but also in their external membrane indicates that differentia- tion of AMSCMBO is possible in endothelial and in pericytal directions. In addition to bone marrow, pluripotent stem cells were also found in other tissues of the adult body - the adipose, muscle, brain, as well as in peripheral blood and blood from cord/placenta. More