Biodegradation of Tritium Labeled Polychlorinated Biphenyls (PCBS) by Local Salt Tolerant Mesophylic Bacillus Strains英文文献资料.docVIP

Journal of Environmental Protection, 2010, 1, 420-425 doi:10.4236/jep.2010.14048 Published Online December 2010 (http://www.SciRP.org/journal/jep) Biodegradation of Tritium Labeled Polychlorinated Biphenyls (PCBs) by Local Salt Tolerant Mesophylic Bacillus Strains Rasulov Bakhtiyor Abdughafurovich 1 , Kim Andrey Andreevich 2 , Lorenz Adrian 3 , Yadgarov Khojiakbar Tashpulatovich 1 1 Institute of Genetics and Plant Experimental Biology, Uzbekistan Academy of Sciences, Tashkent, Uzbekistan; 2 Institute of Nuclear Physics, Uzbekistan Academy of Sciences, Tashkent, Uzbekistan; Environmental Research, Leipzig, Germany. Email: bakhtiyor_1980@mail.ru 3 Department Isotope Biogeochemistry, Helmholtz Centre for Received August 13 , 2010; revised September 8 , 2010; accepted September 13 , 2010. th th th ABSTRACT Salt-resistant Bacillus strains, isolated from agricultural soils in Uzbekistan, were tested for degradation activity to- wards a mixture of polychlorinated biphenyls (PCBs) under aerobic conditions. The study employed the use of tritium labeled PCB congeners and traced the tritium label in cultures with high salt content. The experiments show that most of the selected strains were able to adsorb a part of the radioactivity, indicating transformation of the added PCBs. Gas chromatography demonstrated transformation of PCBs. The radioactive label was removed from several cultures by up to 91%, indicating also mineralization of PCBs. The study suggests that the isolated strains might be good candidates for the bioremediation of contaminated high-salt soils in Uzbekistan and other Central-Asian countries. Keywords: Bacillus, Biodegradation, PCBs, Salinity, Tritium Label 1. Introduction bioaccumulation in the food chains [1,2]. Several bacteria have been described to degrade PCBs under aerobic cond