Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid Comparative Characterization on the Nanoscale英文文献资料.docVIP

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Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid Comparative Characterization on the Nanoscale英文文献资料.doc

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Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid Comparative Characterization on the Nanoscale英文文献资料

HindawiPublishingCorporation JournalofNanotechnology Volume2012,ArticleID954601,9pages doi:10.1155/2012/954601 ResearchArticle BiomolecularTriconjugatesFormedbetweenGold,Protamine, andNucleicAcid:ComparativeCharacterizationontheNanoscale RobertK.DeLong, TiffanySevers, KartikGhosh, AshleySchaeffer, andKristinJ.Flores 1 LisaCillessen, 4 2 ChrisReynolds, 5 3 AdamWanekaya, JohnBlack, 7 3 3 MichaelFisher, StephanieBarber, 6 1 1 1 2 3 4 5 6 7 CellandMolecularBiologyProgram,DepartmentofBiomedicalSciences,MissouriStateUniversity,Spring?eld,MO65897,USA CollegeofPharmacy,TheOhioStateUniversity,Columbus,OH43210,USA DepartmentofChemistry,MissouriStateUniversity,Spring?eld,MO65897,USA DepartmentofPhysicsAstronomyandMaterialsScience,MissouriStateUniversity,Spring?eld,MO65897,USA SchoolofMedicine,UniversityofNorthCarolinaatChapelHill,ChapelHill,NC27599,USA GeorgeWarrenBrownSchoolofSocialWork,WashingtonUniversityinSt.Louis,St.Louis,MO63130,USA SchoolofMedicine,UniversityofPittsburgh,Pittsburg,PA15261,USA CorrespondenceshouldbeaddressedtoRobertK.DeLong,robertdelong@ Received15June2011;Accepted11August2011 AcademicEditor:DongwooKhang Copyright?2012RobertK.DeLongetal.Thisisanopenaccessarticle distributedundertheCreativeCommonsAttribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. DNA and RNA micro- and nanoparticles are increasingly being used for gene and siRNA drug delivery and a variety of otherapplicationsinbionanotechnology.Onthenanoscale,theseentitiesrepresentuniquechallengesfromaphysicochemical characterization perspective. Here, nucleic acid conjugates with protamine and gold nanoparticles (GNP) were characterized comparativelyinthenanorangeofconcentrationbyUV/VisNanoDropspectroscopy,?uorimetry,andgelelectrophoresis.Given theintenseinterestinsplice-siteswitchingoligomers(SSOs),weutilizedahumantumorcellculturesystem(HeLapLuc-705),in whichSSO-directedsplicingrepairupregulatesluciferaseexpression,in