Bioreactor for the reconstitution of a decellularized vascular matrix of biological origin英文文献资料.docVIP

J. Biomedical Science and Engineering, 2011, 4, 435-442 JBiSE doi:10.4236/jbise.2011.46055 Published Online June 2011 (http://www.SciRP.org/journal/jbise/). Bioreactor for the reconstitution of a decellularized vascular matrix of biological origin Matthew G. Geeslin, Gabriel J. Caron, Stefan M. Kren, Ephraim M. Sparrow, David A. Hultman, Doris A. Taylor Department of Mechanical Engineering, University of Minnesota, Minneapolis, USA; Center for Cardiovascular Repair, University of Minnesota, Minneapolis, USA. Email: geesl001@ Received 11 April 2011; revised 1 May 2011; accepted 11 May 2011. ABSTRACT (autologous) cells. Harvesting appropriate cells from a patient, expanding the cells in culture, and applying them to the decellularized matrix, accomplish recellu- larization. Acellular matrices derived from animal and human cadaveric donor vessels or other tubular matrices are appropriate candidates for the creation of tissue- en- gineered, small-diameter, muscular arteries. Engi- neering principles have been used to design a biore- actor and the necessary auxiliary systems for the re- constitution of a previously decellularized vascular matrix. The bioreactor enables the attachment of cells to the luminal and/or exterior surfaces of the matrix. For the recellularization procedure, the ma- trix is situated within a sealed compartment in order to maintain a sterile environment. The matrix is ro- tated continuously to assure a spatially uniform re- constitution. The auxiliary systems that serve the bioreactor are: (a) an oxygenator, (b) peristaltic pumps, one for conveying the internal cell medium and the other for conveying the external cell medium, (c) motor and gearing to create steady and controlled rotation, (d) reservoirs for the containment of the two media, and (e) tubing to convey the respective fluids and to interconnect the bioreactor culture chamber to the v