Blood Compatibility of Amphiphilic Poly(N-α-acrylamide-L-lysine-b-dimethylsiloxane) Block Copolymers英文文献资料.docVIP

Journal of Biomaterials and Nanobiotechnology, 2011, 2, 337-346 337 doi:10.4236/jbnb.2011.24042 Published Online October 2011 (http://www.SciRP.org/journal/jbnb) Blood Compatibility of Amphiphilic Poly(N-α-acrylamide-L-lysine-b-dimethylsiloxane) Block Copolymers Kazuo Sugiyama, Nobuyuki Tanigawa, Kohei Shiraishi Cluster of Biotechnology and Chemistry Systems, Program in Systems Engineering, Graduate School of Systems Engineering, Kinki University, Higashihiroshima-City, Japan. Email: sugiyama@hiro.kindai.ac.jp Received June 21 , 2011; revised July 22 , 2011; accepted August 20 , 2011. st nd th ABSTRACT Amphiphilic block copolymers poly(LysAA-b-DMS) consisting of a hydrophilic poly(N-α-acrylamide-L-lysine) [poly(LysAA)] segment with different molecular weights and a hydrophobic polydimethylsiloxane (PDMS) segment were prepared as follows. The precursor copolymer poly(Boc-LysAA-OtBu-b-PDMS) was obtained from radical polymerization of N-α-acrylamide-N-ε-tert-butoxycarbonyl-L-lysine-tert-butylester (Boc-LysAA-OtBu) initiated with 4,4’-azobis(polydi- 3 methylsiloxane 4-cyanopentanoate) (azo-PDMS) with the molecular weight of PDMS Mw = 4.3 × 10 in the presence of 2-mercaptoethanol (2-ME) as a chain-transfer agent. Removal of the protecting groups of the precursor copolymer was carried out in 80% trifluoroacetic acid aqueous solution to give poly(LysAA-b-DMS)-1-3. The weight average molecu- lar weight of poly(LysAA-b-DMS)-1-3 was Mw = 1.02 × 104 - 2.52 × 104. From the H-NMR and fluorescence spectra 1 measurements, poly(LysAA-b-DMS)-1-3 was determined to self-organize and form core-shell micelles in water. The critical micelle concentration (CMC) increased to 1000 - 4000 mg·L with increasing molar ratio of the poly(LysAA) –1 segment from 0.42 to 0.65. From morphological analysis with a scanning probe microscope