Calcium Imaging of Living Astrocytes in the Mouse Spinal Cord following Sensory Stimulation英文文献资料.docVIP

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Calcium Imaging of Living Astrocytes in the Mouse Spinal Cord following Sensory Stimulation英文文献资料.doc

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Calcium Imaging of Living Astrocytes in the Mouse Spinal Cord following Sensory Stimulation英文文献资料

HindawiPublishingCorporation NeuralPlasticity Volume2012,ArticleID425818,6pages doi:10.1155/2012/425818 ResearchArticle CalciumImagingofLivingAstrocytesin theMouseSpinalCordfollowingSensoryStimulation GiovanniCirillo,DanieleDeLuca,andMichelePapa LaboratoriodiMorfologiadelleRetiNeuronali,DipartimentodiMedicinaPubblicaClinicaePreventiva,SecondaUniversit`adiNapoli, 80100Napoli,Italy CorrespondenceshouldbeaddressedtoMichelePapa,michele.papa@unina2.it Received25July2012;Accepted2September2012 AcademicEditor:EduardKorkotian Copyright?2012GiovanniCirilloetal. This is an open access article distributed under the Creative Commons Attribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. Astrocytic Ca 2+ dynamics have been extensively studied in ex vivo models; however, the recent development of two-photon signalinginlivingcentralnervoussystem.Ca waves 2+ microscopyandastrocyte-speci?clabelinghasallowedthestudyofCa 2+ inastrocyteshavebeendescribedinculturedcellsandslicepreparations,butevidenceforastrocyticactivationduringsensory activityislacking.Therearecurrentlyfewmethodstoimagelivingspinalcord:breathingandheart-beatingartifactshaveimpeded thewidespreadapplicationofthistechnique.Wehereimagedthelivingspinalcordbytwo-photonmicroscopyinC57BL6/Jmice. Throughpressurizedinjection,wespeci?callyloadedspinalastrocytesusingthered?uorescentdyesulforhodamine101(SR101) andimagedastrocyticCa 2+ 2+ levelswithOregon-GreenBAPTA-1(OGB).Then,westudiedastrocyticCa levelsatrestandafter rightelectricalhindpawstimulation.Sensorystimulationsigni?cantlyincreasedastrocyticCa 2+ levelswithinthesuper?cialdorsal hornofthespinalcordcomparedtorest.Inconclusion,invivomorphofunctionalimagingoflivingastrocytesinspinalcord revealedthatastrocytesactivelyparticipatetosensorystimulation. 1.Introduction investigated[20,21],buttoolsallowingimagingofglialcells inspinalcordinvivoarejustemerging[22]. In this work, we have performed t