Can Replication Save Noisy Microarray Data英文文献资料.docVIP

ComparativeandFunctionalGenomics CompFunctGenom2002;3 :369–371. PublishedonlineinWileyInterScience().DOI:10.1002/cfg.197 ConferenceReview BμG@Sbase—amicroarraydatabaseand analysistool AdamA.Witney*andJasonHinds Bacterial Microarray Group,Department ofMedicalMicrobiology, StGeorge’s Hospital MedicalSchool,Cranmer Terrace,LondonSW17 0RE,UK *Correspondence to: Abstract AdamA.Witney,Bacterial Microarray Group,Department ofMedicalMicrobiology, St George’s Hospital Medical Themanufacture anduseofawhole-genome microarrayisacomplexprocessanditis essentialthatalldatasurrounding theprocessisstored,isaccessible andcanbeeasily associated with the data generated following hybridization and scanning. As part of School,Cranmer Terrace,London a program funded by the Wellcome Trust, the Bacterial Microarray Group at St. SW170RE,UK. E-mail:a.witney@sghms.ac.uk George’s HospitalMedicalSchool(BμG@S)willgeneratewhole-genome microarrays for 12 bacterial pathogens for use in collaboration with specialist research groups. BμG@S will collaborate with these groups at all levels, including the experimental design, methodology and analysis. In addition, we will provide informatic support in the form of a database system (BμG@Sbase). BμG@Sbase will provide access through a web interface to the microarray design data and will allow individual users tostoretheir data inasearchable, secure manner. Toolsdeveloped byBμG@S incollaboration withspeci?c researchgroupsinvestigating analysis methodology will also be made available to those groups using the arrays and submitting data to BμG@Sbase. Copyright ?2002 John Wiley Sons, Ltd. Received: 10June2002 Accepted: 12June2002 Keywords: microarray; bioinformatics; database Introduction useful during the analysis phase of a microarray experiment; it is helpful to know which portion of a gene has been ampli?ed and printed onto the slide, and this becomes crucial when identifying genedeletionsbyDNAagainstDNAhybridization comparisons. Also, the