A Consensus Microsatellite-Based Linkage Map for the Hermaphroditic Bay Scallop (Argopecten irradians) and Its Application in Size-Related QTL Analysis 英文参考文献.docVIP

AConsensusMicrosatellite-BasedLinkageMapforthe HermaphroditicBayScallop(Argopectenirradians)and ItsApplicationinSize-RelatedQTLAnalysis HongjunLi¤,XiaoLiu*,GuofanZhang InstituteofOceanology,ChineseAcademyofSciences,Qingdao,China Abstract Bayscallop(Argopectenirradians)isoneofthemosteconomicallyimportantaquaculturespeciesinChina.Inthisstudy,we constructedaconsensusmicrosatellite-basedgeneticlinkagemapwithamappingpanelcontainingtwohybridbackcross- like families involving two subspecies of bay scallop, A. i. irradians and A. i. concentricus. One hundred sixty-one microsatelliteandonephenotypic(shellcolor)markersweremappedto16linkagegroups(LGs),whichcorrespondstothe haploidchromosomenumberofbayscallop.Thesex-specificmapwas779.2cMand781.6cMlonginfemaleandmale, respectively,whereasthesex-averagedmapspanned849.3cM.Theaverageresolutionofintegratedmapwas5.9cM/locus and the estimated coverage was 81.3%. The proportion of distorted markers occurred more in the hybrid parents, suggestingthatthesegregationdistortionwaspossiblyresultedfromheterospecificinteractionbetweengenomesoftwo subspeciesofbayscallop.Theoverallfemale-to-malerecombinationratewas1.13:1acrossalllinkedmarkersincommonto both parents, and considerable differences in recombination also existed among different parents in both families. Four size-relatedtraits,includingshelllength(SL),shellheight(SH),shellwidth(SW)andtotalweight(TW)weremeasuredfor quantitativetraitloci(QTL)analysis.ThreesignificantandsixsuggestiveQTLweredetectedonfiveLGs.Amongthethree significant QTL, two (qSW-10 and qTW-10, controlling SW and TW, respectively) were mapped on the same region near markerAiAD121onLG10andexplained20.5%and27.7%ofthephenotypicvariance,whilethethird(qSH-7,controllingSH) was located on LG7 and accounted for 15.8% of the phenotypic variance. Six suggestive QTL were detected on four differentLGs.Thelinkagemapandsize-relatedQTLobtainedinthisstudymayfacilitatemarker-assistedselection(MAS)in bayscallop. Citation:LiH,LiuX,Zhang