Adenoviruses in Lymphocytes of the Human Gastro-Intestinal Tract 英文参考文献.docVIP

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Adenoviruses in Lymphocytes of the Human Gastro-Intestinal Tract 英文参考文献.doc

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Adenoviruses in Lymphocytes of the Human Gastro-Intestinal Tract 英文参考文献

AdenovirusesinLymphocytesoftheHumanGastro- IntestinalTract SoumitraRoy¤,RobertoCalcedo,AngelicaMedina-Jaszek,MartinKeough,HuiPeng,JamesM.Wilson* Gene Therapy Program, Department of Pathology and Laboratory Medicine, Division of Transfusion Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, UnitedStatesofAmerica Abstract Objective: Persistent adenoviral shedding in stools is known to occur past convalescence following acute adenoviral infections.Wewishedtoestablishthefrequencywithwhichadenovirusesmaycolonizethegutinnormalhumansubjects. Methods:ThepresenceofadenoviralDNAinintestinalspecimensobtainedatsurgeryorautopsywastestedusinganested PCR method. The amplified adenoviral DNA sequences were compared to each other and to known adenoviral species. Laminaproprialymphocytes(LPLs)wereisolatedfromthespecimensandtheadenoviralcopynumbersintheCD4+and CD8+fractionsweredeterminedbyquantitativePCR.Adenoviralgeneexpressionwastestedbyamplificationofadenoviral mRNA. Results:Intestinaltissuefrom21of58donorsandLPLsfrom21of24donorswerepositiveforthepresenceofadenoviral DNA.ThemajorityofthesequencescouldbeassignedtoadenoviralspeciesE,althoughspeciesBandCsequenceswere also common. Multiple sequences were often present in the same sample. Forty-one non-identical sequences were identifiedfrom39differenttissuedonors.QuantitativePCRforadenoviralDNAinCD4+andCD8+fractionsofLPLsshowed adenoviralDNAtobepresentinbothcelltypesandrangedfromafewhundredtoseveralmillioncopiespermillioncellson average. Active adenoviral gene expression as evidenced by the presence of adenoviral messenger RNA in intestinal lymphocyteswasdemonstratedin9ofthe11donorstested. Conclusion:AdenoviralDNAishighlyprevalentinlymphocytesfromthegastro-intestinaltractindicatingthatadenoviruses maybepartofthenormalgutflora. Citation:RoyS,CalcedoR,Medina-JaszekA,KeoughM,PengH,etal.(2011)AdenovirusesinLymphocytesoftheHumanGastro-IntestinalTract.PLoSONE6(9): e24859.doi:10.1371/journal.pone.0024859 Editor:EricJ.Kremer,F