Antibodies for Assessing Circadian Clock Proteins in the Rodent Suprachiasmatic Nucleus 英文参考文献.docVIP
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Antibodies for Assessing Circadian Clock Proteins in the Rodent Suprachiasmatic Nucleus 英文参考文献
AntibodiesforAssessingCircadianClockProteinsinthe
RodentSuprachiasmaticNucleus
JosephLeSauter1,ChristopherM.Lambert2,MargaretR.Robotham1,ZinaModel1,RaeSilver1,3,4,
DavidR.Weaver2*
1Department of Psychology, Barnard College of Columbia University, New York, New York, United States of America, 2Department of Neurobiology, University of
MassachusettsMedicalSchool,Worcester,Massachusetts,UnitedStatesofAmerica,3DepartmentofPsychology,ColumbiaUniversity,NewYork,NewYork,UnitedStates
ofAmerica,4DepartmentofPathologyandCellBiology,ColumbiaUniversityHealthSciences,NewYork,NewYork,UnitedStatesofAmerica
Abstract
Researchonthemechanismsunderlyingcircadianrhythmicityandtheresponseofbrainandbodyclockstoenvironmental
andphysiologicalchallengesrequiresassessinglevelsofcircadianclockproteins.Toooften,however,itisdifficulttoacquire
antibodiesthatspecificallyandreliablylabeltheseproteins.Manyoftheseantibodiesalsolackappropriatevalidation.The
goalofthisprojectwastogenerateandcharacterizeantibodiesagainstseveralcircadianclockproteins.Weexaminedmice
andhamstersatpeakandtroughtimesofclockproteinexpressioninthesuprachiasmaticnucleus(SCN).Inaddition,we
confirmedspecificitybytestingtheantibodiesonmicewithtargeteddisruptionoftherelevantgenes.Ourresultsidentify
antibodies against PER1, PER2, BMAL1 and CLOCK that are useful for assessing circadian clock proteins in the SCN by
immunocytochemistry.
Citation:LeSauterJ,LambertCM,RobothamMR,ModelZ,SilverR,etal.(2012)AntibodiesforAssessingCircadianClockProteinsintheRodentSuprachiasmatic
Nucleus.PLoSONE7(4):e35938.doi:10.1371/journal.pone.0035938
Editor:ShinYamazaki,VanderbiltUniversity,UnitedStatesofAmerica
ReceivedJanuary29,2012;AcceptedMarch27,2012;PublishedApril27,2012
Copyright: ? 2012 LeSauter et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited.
Funding:Thisworkwassupporte
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