Association of Calcineurin with the COPI Protein Sec28 and the COPII Protein Sec13 Revealed by Quantitative Proteomics 英文参考文献.docVIP
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Association of Calcineurin with the COPI Protein Sec28 and the COPII Protein Sec13 Revealed by Quantitative Proteomics 英文参考文献
AssociationofCalcineurinwiththeCOPIProteinSec28
andtheCOPIIProteinSec13RevealedbyQuantitative
Proteomics
LukaszKozubowski1*,J.WillThompson2,MariaE.Cardenas1,M.ArthurMoseley2,JosephHeitman1
1Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America, 2Duke Proteomics Core
Facility,DukeUniversityMedicalCenter,Durham,NorthCarolina,UnitedStatesofAmerica
Abstract
Calcineurin is a calcium-calmodulin-dependent serine/threonine specific protein phosphatase operating in key cellular
processesgoverningresponsestoextracellularcues.Calcineurinisessentialforgrowthathightemperatureandvirulenceof
thehumanfungalpathogenCryptococcusneoformansbuttheunderlyingmechanismisunknown.Weperformedamass
spectrometryanalysistoidentifyproteinsthatassociatewiththecalcineurinAcatalyticsubunit(Cna1)inC.neoformanscells
grownundernon-stressandhightemperaturestressconditions.Anovelprioritizationstrategyformassspectrometrydata
fromimmunoprecipitationexperimentsidentifiedputativesubstratesandproteinspotentiallyoperatingwithcalcineurinin
commonpathways.Cna1co-purifiedwithproteinsinvolvedinmembranetraffickingincludingtheCOPIcomponentSec28
andtheCOPIIcomponentSec13.TheassociationofCna1withSec28andSec13wasconfirmedbyco-immunoprecipitation.
Cna1exhibitedadramaticchangeinsubcellularlocalizationduringhightemperaturestressfromdiffusecytoplasmictoER-
associatedpunctaandthemother-budneckandco-localizedwithSec28andSec13.
Citation:KozubowskiL,ThompsonJW,CardenasME,MoseleyMA,HeitmanJ(2011)AssociationofCalcineurinwiththeCOPIProteinSec28andtheCOPIIProtein
Sec13RevealedbyQuantitativeProteomics.PLoSONE6(10):e25280.doi:10.1371/journal.pone.0025280
Editor:Yong-SunBahn,YonseiUniversity,RepublicofKorea
ReceivedJuly11,2011;AcceptedAugust30,2011;PublishedOctober3,2011
Copyright:?2011Kozubowskietal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermits
unrestricteduse,distribution,andreproductioninanymedium
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