Axonal Varicosity Density as an Index of Local Neuronal Interactions 英文参考文献.docVIP

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Axonal Varicosity Density as an Index of Local Neuronal Interactions 英文参考文献.doc

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Axonal Varicosity Density as an Index of Local Neuronal Interactions 英文参考文献

AxonalVaricosityDensityasanIndexofLocalNeuronal Interactions Zi-WeiZhang1,2,JunIlKang1,2,ElvireVaucher1* 1SchoolofOptometry,Universite′ deMontre′al,Montre′al,Quebec,Canada,2DepartmentofPhysiology,Universite′ deMontre′al,Montre′al,Quebec,Canada Abstract Diffuse transmission is an important non-synaptic communication mode in the cerebral neocortex, in which neurotransmitters released from en passant varicosities interact with surrounding cells. In a previous study we have shown that the cholinergic axonal segments which were in the microproximity with dopaminergic fibers possessed a greaterdensityofenpassantvaricositiescomparedtomoredistantsegments,suggestinganactivity-dependentlevelofen passantvaricositiesintheaxonalzoneofinteraction.Tofurtherevaluatethisplasticrelationship,thedensityofcholinergic varicositieswasquantifiedonfibersegmentswithinthemicroproximityofactivatedornon-activatedpyramidalcellsofthe prefrontal cortex (mPFC). Repetitive 14 days patterned visual stimulation paired with an electrical stimulation of the cholinergicfibersprojectingtothemPFCfromtheHDBwasperformedtoinducepersistentaxonalplasticchanges.Thec- Fos early gene immunoreactivity was used as a neuronal activity marker of layer V pyramidal cells, labelled with anti- glutamatetransporterEAAC1.Cholinergicfiberswerelabeledwithanti-ChAT(cholineacetyltransferase)immunostaining. ThedensityofChAT+varicositiesonandthelengthoffibersegmentswithinthe3 mmmicroproximityofc-Fospositive/ negativepyramidalcellswereevaluatedonconfocalimages.Morethan50%ofthepyramidalcellsinthemPFCwerec-Fos immunoreactive.DensityofChAT+varicositieswassignificantlyincreasedwithin3mmvicinityofactivatedpyramidalcells (0.5060.01permmofChAT+fiberlength)comparedtonon-activatedcellsinthisgroup(0.3460.001;p#0.05)orcontrol rats(0.3260.02;p#0.05).Differenttypesofstimulation(visual,HDBorvisual/HDB)inducedsimilarincreaseofthedensityof ChAT+varicositieswithinmicroproximityofactivatedpyramidalcells.Thisstudydemonstratedatthe