Biophysical Characterization of the Strong Stabilization of the RNA Triplex poly(U)？poly(A)poly(U) by 9-O-(ω-amino) Alkyl Ether Berberine Analogs 英文参考文献.docVIP

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Biophysical Characterization of the Strong Stabilization of the RNA Triplex poly(U)？poly(A)poly(U) by 9-O-(ω-amino) Alkyl Ether Berberine Analogs 英文参考文献.doc

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Biophysical Characterization of the Strong Stabilization of the RNA Triplex poly(U)？poly(A)poly(U) by 9-O-(ω-amino) Alkyl Ether Berberine Analogs 英文参考文献

BiophysicalCharacterizationoftheStrongStabilization oftheRNATriplexpoly(U)Npoly(A)*poly(U)by9-O-(v- amino)AlkylEtherBerberineAnalogs DebipreetaBhowmik1.,SumanDas2.,MaidulHossain1,LucyHaq2,GopinathaSureshKumar1* 1BiophysicalChemistryLaboratory,ChemistryDivision,CSIR-IndianInstituteofChemicalBiology,Kolkata,India,2DepartmentofChemistry,JadavpurUniversity,Kolkata, India Abstract Background: Binding of two 9-O-(v-amino) alkyl ether berberine analogs BC1 and BC2 to the RNA triplex poly(U)Npoly(A)*poly(U)wasstudiedbyvariousbiophysicaltechniques. Methodology/PrincipalFindings:BerberineanalogsbindtotheRNAtriplexnon-cooperatively.Theaffinityofbindingwas remarkablyhighbyabout5and15times,respectively,forBC1andBC2comparedtoberberine.Thesitesizeforthebinding was around 4.3 for all. Based on ferrocyanide quenching, fluorescence polarization, quantum yield values and viscosity resultsastrongintercalativebindingofBC1andBC2totheRNAtriplexhasbeendemonstrated.BC1andBC2stabilizedthe Hoogsteenbasepairedthirdstrandbyabout18.1and20.5uCcomparedtoa17.5uCstabilizationbyberberine.Thebinding wasentropydrivencomparedtotheenthalpydrivenbindingofberbeine,mostlikelyduetoadditionalcontactswithinthe groovesofthetriplexanddisruptionofthewaterstructurebythealkylsidechain. Conclusions/Significance:RemarkablyhigherbindingaffinityandstabilizationeffectoftheRNAtriplexbytheaminoalkyl berberine analogs was achieved compared to berberine. The length of the alkyl side chain influence in the triplex stabilizationphenomena. Citation: Bhowmik D, Das S, Hossain M, Haq L, Suresh Kumar G (2012) Biophysical Characterization of the Strong Stabilization of the RNA Triplex poly(U)Npoly(A)*poly(U)by9-O-(v-amino)AlkylEtherBerberineAnalogs.PLoSONE7(5):e37939.doi:10.1371/journal.pone.0037939 Editor:HaiweiSong,InstituteofMolecularandCellBiology,Singapore ReceivedMarch7,2012;AcceptedApril26,2012;PublishedMay29,2012 Copyright: ? 2012 Bhowmik et al. This is an open-access article distributed under the terms of the Creati