MTG酶催化羊毛固定化溶菌酶的酶学性质及抗菌性能.pdfVIP

学兔兔 化 工 进 展 2009年第28卷第7期 CHEMICAL INDUSTRY AND ENGINEERE G PROGRESS ·1231· MTG酶催化羊毛固定化溶菌酶的酶学性质及抗菌性能 黄 栋，范雪荣，崔 莉，王 强，王 平 (江南大学 生态纺织教育部重点实验室，江苏 无锡214122) 摘 要：利用新型的生物催化剂微生物谷氨酰胺转胺酶 (MTG)催化溶菌酶在羊毛织物上的固定化。采用 SDS．PAGE法探讨了溶菌酶作为MTG催化底物的可行性以及固定化溶菌酶的酶争}生质及抗菌性能。研究结果表 明，羊毛织物经过高锰酸钾预处理后可以实现溶菌酶的固定化。固定化酶的pH值稳定性和温度稳定性优于游离 酶；与物理吸附法相比，MTG催化法可赋予固定化溶菌酶的羊毛织物更好的耐水洗性和操作稳定性；此外，固定 化酶的低温 (4℃)干储藏稳定性良好，30天内酶活仍维持在80％}X上。抗菌测试结果表明，MTG催化法固定 化溶菌酶的羊毛织物对金黄色葡萄球菌的抑菌率达73．23％，抗菌效果良好。 关键词：微生物谷氨酰胺转胺酶；羊毛；固定化；溶菌酶；酶学性质；抗茵性能 中图分类号：TS 131．9 文献标志码：A 文章编号：1000—6613(2009)07—1231—06 Antibacterial action and properties of lysozyme immobilized on wool catalyzed by MTG HUANGDong，FANXuerong，CUILi，WANGQiang，WANGPf (Key Laboratory of Science and Technology of Eco—Textile，Ministry of Education。Jiangnan University，Wuxi 214122， Jiangsu，C~na) Abstract：A new biocatalyst microbial transglutaminase(MTG)was used to immobilize lysozyme on wool fabrics．In this process，sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS—PAGE) was used to verify that MTG can catalyze lysozyme．Besides，the enzymology characteristics and antibacterial performance of imm obilized lysozyme was discussed．The result showed that lysozyme could be imm obilized on wool pretreated by potassium perm anganate．Compared with free lysozyme， the immobilized enzyme showed better stability to pH and temperature．Lysozyme imm obilized on wool catalyzed by MTG had beRer washing and operational stability than that imm obilized only by adsorption． In addition，storage stability of immobilized lysozyme was better in dry condition at