Canine Uterine Bacterial Infection Induces Upregulation of Proteolysis-Related Genes and Downregulation of Homeobox and Zinc Finger Factors 英文参考文献.docVIP

CanineUterineBacterialInfectionInducesUpregulation ofProteolysis-RelatedGenesandDownregulationof HomeoboxandZincFingerFactors RagnviHagman1*,ElinRo¨nnberg2,GunnarPejler2* 1DivisionofSmallAnimals,DepartmentofClinicalSciences,SwedishUniversityofAgriculturalSciences,Uppsala,Sweden, 2DepartmentofAnatomy,Physiologyand Biochemistry,SwedishUniversityofAgriculturalSciences,Uppsala,Sweden Abstract Background:Bacterialinfectionwiththeseverecomplicationofsepsisisafrequentandseriouscondition,beingamajor causeofdeathworldwide.Tocopewiththeplethoraofoccurringbacterialinfectionsthereisthereforeanurgentneedto identify molecular mechanisms operating during the host response, in order both to identify potential targets for therapeutic intervention and to identify biomarkers for disease. Here we addressed this issue by studying global gene expressioninuterifromfemaledogssufferingfromspontaneouslyoccurringuterinebacterialinfection. Principal Findings: The analysis showedthat almost 800genes weresignificantly (p,0.05) upregulated (.2-fold) in the uteriofdiseasedanimals.Amongthesewerenumerouschemokineandcytokinegenes,aswellasgenesassociatedwith inflammatory cell extravasation, anti-bacterial action, the complement system and innate immune responses, as well as proteoglycan-associated genes. There was also a striking representation of genes associated with proteolysis. Robust upregulation of immunoglobulin components and genes involved in antigen presentation was also evident, indicating elaboration of a strong adaptive immune response. The bacterial infection was also associated with a significant downregulation of almost 700 genes, of which various homeobox and zinc finger transcription factors were highly represented. Conclusions/Significance: Together, these finding outline the molecular patterns involved in bacterial infection of the uterus.Thestudyidentifiedalteredexpressionofnumerousgenesnotpreviouslyimplicatedinbacterialdisease,andseveral ofthesemaybeevaluatedforpotentia