Differences in Salivary Alpha-Amylase and Cortisol Responsiveness following Exposure to Electrical Stimulation versus the Trier Social Stress Tests 英文参考文献.docVIP

DifferencesinSalivaryAlpha-AmylaseandCortisol ResponsivenessfollowingExposuretoElectrical StimulationversustheTrierSocialStressTests YoshihiroMaruyama,AimiKawano,ShizukoOkamoto,TomokoAndo,YoshinobuIshitobi, YoshihiroTanaka,AyakoInoue,JunkoImanaga,MasayukiKanehisa,HarukaHiguma,TaigaNinomiya, JusenTsuru,HiroakiHanada,JotaroAkiyoshi* Department of Neuropsychiatry, Oita University Faculty of Medicine, Yufu-Shi, Oita, Japan Abstract Background: Cortisol is an essential hormone in the regulation of the stress response along the HPA axis, and salivary cortisol has been used as a measure of free circulating cortisol levels. Recently, salivary alpha-amylase (sAA) has also emerged as a novel biomarker for psychosocial stress responsiveness within the sympathetic adrenomedullary (SAM) system. PrincipalFindings: We measured sAA and salivary cortisol in healthy volunteers after exposure to the Trier Social Stress Test (TSST) and electric stimulation stress. One hundred forty-nine healthy volunteers participated in this study. All subjects were exposed to both the TSST and electric stimulation stress on separate days. We measured sAA and salivary cortisol levels three times immediately before, immediately after, and 20 min after the stress challenge. The State (STAI-S) and Trait (STAI- T) versions of the Spielberger Anxiety Inventory test and the Profile of Mood State (POMS) tests were administered to participants before the electrical stimulation and TSST protocols. We also measured HF, LF and LF/HF Heart Rate Variability ratio immediately after electrical stimulation and TSST exposure. Following TSST exposure or electrical stimulation, sAA levels displayed a rapid increase and recovery, returning to baseline levels 20 min after the stress challenge. Salivary cortisol responses showed a delayed increase, which remained significantly elevated from b