Directed Evaluation of Enterotoxigenic Escherichia coli Autotransporter Proteins as Putative Vaccine Candidates 英文参考文献.docVIP

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Directed Evaluation of Enterotoxigenic Escherichia coli Autotransporter Proteins as Putative Vaccine Candidates 英文参考文献.doc

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Directed Evaluation of Enterotoxigenic Escherichia coli Autotransporter Proteins as Putative Vaccine Candidates 英文参考文献

DirectedEvaluationofEnterotoxigenicEscherichiacoli AutotransporterProteinsasPutativeVaccineCandidates JessicaA.Harris1.,KoushikRoy2.,VirginiaWoo-Rasberry3,DavidJ.Hamilton4,RitaKansal3 ,Firdausi Qadri5,JamesM.Fleckenstein2,6,7* 1UniversityofTennesseeCollegeofMedicine,Memphis,Tennessee,UnitedStatesofAmerica,2DepartmentofMedicine,UniversityofTennesseeHealthSciencesCenter, Memphis,Tennessee,UnitedStatesofAmerica, 3ResearchServices,VeteransAffairs MedicalCenter,Memphis,Tennessee,United StatesofAmerica, 4Department of Comparative Medicine, University of Tennessee Health Sciences Center, Memphis, Tennessee, United States of America, 5International Centre for Diarrheal Disease Research, Dhaka, Bangladesh, 6Department of Molecular Sciences, University of Tennessee Health Sciences Center, Memphis, Tennessee, United States of America, 7DepartmentofMedicine,VeteransAffairsMedicalCenter,Memphis,Tennessee,UnitedStatesofAmerica Abstract Background: Enterotoxigenic Escherichia coli (ETEC) is a major diarrheal pathogen in developing countries, where it accountsformillionsofinfectionsandhundredsofthousandsofdeathsannually.Whilevaccinedevelopmenttoprevent diarrheal illness due to ETEC is feasible, extensive effort is needed to identify conserved antigenic targets. Pathogenic Escherichiacoli,includingETEC,usetheautotransporter(AT)secretionmechanismtoexportvirulencefactors.ATproteins arecomprisedofahighlyconservedcarboxyterminaloutermembranebetabarrelandasurface-exposedaminoterminal passenger domain. Recent immunoproteomic studies suggesting that multiple autotransporter passenger domains are recognizedduringETECinfectionpromptedthepresentstudies. Methodology:AvailableETECgenomeswereexaminedtoidentifyATcodingsequencespresentinpathogenicisolates,but not in the commensal E. coli HS strain. Passenger domains of the corresponding autotransporters were cloned and expressedasrecombinantantigens,andtheimmuneresponsetotheseproteinswasthenexaminedusingconvalescent serafrompatientsandexp