DNA from KI, WU and Merkel Cell Polyomaviruses Is Not Detected in Childhood Central Nervous System Tumours or Neuroblastomas 英文参考文献.docVIP

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DNA from KI, WU and Merkel Cell Polyomaviruses Is Not Detected in Childhood Central Nervous System Tumours or Neuroblastomas 英文参考文献.doc

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DNA from KI, WU and Merkel Cell Polyomaviruses Is Not Detected in Childhood Central Nervous System Tumours or Neuroblastomas 英文参考文献

DNAfromKI,WUandMerkelCellPolyomavirusesIsNot DetectedinChildhoodCentralNervousSystemTumours orNeuroblastomas Ge′raldineGiraud1*,Torbjo¨rnRamqvist1,DianaV.Pastrana2,VincentPavot1,CeciliaLindau3, PerKogner4,AbielOrrego1,ChristopherB.Buck2,TobiasAllander3,StefanHolm4,BengtGustavsson5, TinaDalianis1,6 1DepartmentofOncology-Pathology,KarolinskaInstitutet,Stockholm,Sweden,2LaboratoryofCellularOncology,CenterforCancerResearch,NationalCancerInstitute, Bethesda,Maryland,UnitedStatesofAmerica,3DepartmentofMicrobiology,TumorandCellBiology,KarolinskaInstitutet,Stockholm,Sweden,4DepartmentofWomen andChildHealth,KarolinskaInstitutet,Stockholm,Sweden,5DepartmentofNeurosciences,KarolinskaInstitutet,Stockholm,Sweden,6DepartmentofVirology,Swedish InstituteforInfectiousDiseaseControl,Solna,Sweden Abstract Background:BKandJCpolyomaviruses(BKVandJCV)arepotentiallyoncogenicandhaveinthepastinconclusivelybeen associated with tumours of the central nervous system (CNS), while BKV has been hinted, but not confirmed to be associatedwithneuroblastomas.Recentlythreenewpolyomaviruses(KIPyV,WUPyVandMCPyV)wereidentifiedinhumans. So far KIPyV and WUPyV have not been associated to human diseases, while MCPyV was discovered in Merkel Cell carcinomas and may have neuroepithelial cell tropism. However, all three viruses can be potentially oncogenic and this compelledustoinvestigatefortheirpresenceinchildhoodCNSandneuroblastomas. Methodology:ThepresenceofKI,WUandMCPyVDNAwasanalysed,byajointWUandKIspecificPCR(coveringpartof VP1)andbyaMCPyVspecificregularandrealtimequantitativePCR(coveringpartofLargeT)in25CNStumourbiopsies and 31 neuroblastoma biopsies from the Karolinska University Hospital, Sweden. None of the three new human polyomaviruseswerefoundtobeassociatedwithanyofthetumours,despitethepresenceofPCRamplifiableDNAassayed byaS14housekeepinggenePCR. Conclusion: In this pilot study, the presence of MCPyV, KI and WU was not observed in childhood CNS tumours and neuroblastomas. Nonetheless, we suggest