Dolabelladienetriol, a Compound from Dictyota pfaffii Algae, Inhibits the Infection by Leishmania amazonensis 英文参考文献.docVIP

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Dolabelladienetriol, a Compound from Dictyota pfaffii Algae, Inhibits the Infection by Leishmania amazonensis 英文参考文献.doc

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Dolabelladienetriol, a Compound from Dictyota pfaffii Algae, Inhibits the Infection by Leishmania amazonensis 英文参考文献

Dolabelladienetriol,aCompoundfromDictyotapfaffii Algae,InhibitstheInfectionbyLeishmaniaamazonensis DeividCostaSoares1,TeresaC.Calegari-Silva2,UlissesG.Lopes2,Vale′riaL.Teixeira3, IzabelC.N.dePalmerPaixa?o4,ClaudioCirne-Santos5,DumithChequerBou-Habib6,ElviraM.Saraiva1* 1DepartamentodeImunologia,InstitutodeMicrobiologiaPaulodeGo′es,UniversidadeFederaldoRiodeJaneiro,RiodeJaneiro,Brazil,2Laborato′riodeParasitologia Molecular, Instituto deBiof?′sica Carlos ChagasFilho, UniversidadeFederal do Rio de Janeiro, Rio de Janeiro, Brazil, 3Departamento de Biologia Marinha, Instituto de Biologia,UniversidadeFederalFluminense,Nitero′i,Brazil,4DepartamentodeBiologiaCelulareMolecular,InstitutodeBiologia,UniversidadeFederalFluminense,Nitero′i, Brazil, 5Laborato′rio de Imunologia Cl?′nica, Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, Brazil, 6Laborato′rio de Pesquisa sobre o Timo, Instituto Oswaldo Cruz/ FIOCRUZ,RiodeJaneiro,Brazil Abstract Background: Chemotherapy for leishmaniasis, a disease caused by Leishmania parasites, is expensive and causes side effects.Furthermore,parasiteresistanceconstitutesanincreasingproblem,andnewdrugsagainstthisdiseaseareneeded. In this study, we examine the effect of the compound 8,10,18-trihydroxy-2,6-dolabelladiene (Dolabelladienetriol), on Leishmaniagrowthinmacrophages.Theabilityofthiscompoundtomodulatemacrophagefunctionisalsodescribed. Methodology/Principal Findings: Leishmania-infected macrophages were treated with Dolabelladienetriol, and parasite growth was measured using an infectivity index. Nitric oxide (NO), TNF-a and TGF-b production were assayed in macrophagesusingspecificassays.NF-kBnucleartranslocationwasanalyzedbywesternblot.Dolabelladienetriolinhibited Leishmania in a dose-dependent manner; the IC50 was 44mM. Dolabelladienetriol diminished NO, TNF-a and TGF-b productioninuninfectedandLeishmania-infectedmacrophagesandreducedNF-kBnucleartranslocation.Dolabelladiene- triolinhibitedLeishmaniainfectionevenwhentheparasitegrowthwasexacer