Duodenal Enteroendocrine I-Cells Contain mRNA Transcripts Encoding Key Endocannabinoid and Fatty Acid Receptors 英文参考文献.docVIP

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Duodenal Enteroendocrine I-Cells Contain mRNA Transcripts Encoding Key Endocannabinoid and Fatty Acid Receptors 英文参考文献.doc

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Duodenal Enteroendocrine I-Cells Contain mRNA Transcripts Encoding Key Endocannabinoid and Fatty Acid Receptors 英文参考文献

DuodenalEnteroendocrineI-CellsContainmRNA TranscriptsEncodingKeyEndocannabinoidandFatty AcidReceptors AlexandrosG.Sykaras1,2,ClaireDemenis1,R.MaynardCase1,JohnT.McLaughlin3,CraigP.Smith1* 1FacultyofLifeSciences,TheUniversityofManchester,Manchester,UnitedKingdom,2GraduateProgramme‘‘MolecularBasisofHumanDiseases’’,FacultyofMedicine, UniversityofCrete,Heraklion,Crete,Greece,3SchoolofTranslationalMedicine,SchoolofMedicine,TheUniversityofManchester,Manchester,UnitedKingdom Abstract Enteroendocrine cells have a critical role in regulation of appetite and energy balance. I-cells are a subtype of enteroendocrine cells localized in duodenum that release cholecystokinin in response to ingested fat and amino-acids. Despitetheirpotentiallypivotalroleinnutrientsensingandfeedingbehaviour,nativeI-cellshavepreviouslybeendifficult toisolateandstudy.HerewedescribearobustprotocolfortheisolationandcharacterizationofnativeduodenalI-cellsand additionally, using semi-quantitative RT-PCR we determined that mouse duodenal I-cells contain mRNA transcripts encoding key fatty acid and endocannabinoid receptors including the long chain fatty acid receptors GPR40/FFAR1, GPR120/O3FAR1;shortchainfattyacidreceptorsGPR41/FFAR3andGPR43/FFAR2;theoleoylethanolamidereceptorGPR119 andtheclassicendocannabinoidreceptorCB1.ThesedatasuggestthatI-cellssenseawiderangeofgutlumennutrients andalsohavethecapacitytorespondtosignalsoffatty-acidderivativesorendocannabinoidpeptides. Citation: Sykaras AG, Demenis C, Case RM, McLaughlin JT, Smith CP (2012) Duodenal Enteroendocrine I-Cells Contain mRNA Transcripts Encoding Key EndocannabinoidandFattyAcidReceptors.PLoSONE7(8):e42373.doi:10.1371/journal.pone.0042373 Editor:NigelIrwin,UniversityofUlster,UnitedKingdom ReceivedApril4,2012;AcceptedJuly4,2012;PublishedAugust2,2012 Copyright: ? 2012 Sykaras et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninany