Dynamics of Brassinosteroid Response Modulated by Negative Regulator LIC in Rice 英文参考文献.docVIP

DynamicsofBrassinosteroidResponseModulatedby NegativeRegulatorLICinRice CuiZhang1,2,YunyuanXu1,SiyiGuo1,2,JiayingZhu1,2,QingHuan1,2,HuanhuanLiu1,2,LeiWang1, GuanzhengLuo3,XiujieWang3,KangChong1,4* 1KeyLaboratoryofPlantMolecularPhysiology/PhotosynthesisandEnvironmentalMolecularPhysiology,InstituteofBotany,ChineseAcademyofSciences,Beijing,China, 2Graduate University of the Chinese Academy of Sciences, Beijing, China, 3Center for Molecular Systems Biology, Institute of Genetics and Developmental Biology, ChineseAcademyofSciences,Beijing,China,4NationalPlantGeneResearchCenter,Beijing,China Abstract Brassinosteroids(BRs)regulatericeplantarchitecture,includingleafbending,whichaffectsgrainyield.AlthoughBRsignaling hasbeeninvestigatedinArabidopsisthaliana,thecomponentsnegativelyregulatingthispathwayarelesswellunderstood. Here, we demonstrate that Oryza sativa LEAF and TILLER ANGLE INCREASED CONTROLLER (LIC) acts as an antagonistic transcriptionfactorofBRASSINAZOLE-RESISTANT1(BZR1)toattenuatetheBRsignalingpathway.Thegain-of-functionmutant lic-1andLIC–overexpressinglinesshowederectleaves,similartoBZR1–depletedlines,whichindicatestheoppositerolesofLIC and BZR1 in regulating leaf bending. Quantitative PCR revealed LIC transcriptionrapidly induced by BR treatment. Image analysisandimmunoblottingshowedthatuponBRtreatmentLICproteinstranslocatefromthecytoplasmtothenucleusina phosphorylation-dependentfashion.PhosphorylationassayinvitrorevealedLICphosphorylatedbyGSK3–likekinases.For negative feedback, LIC bound to the core element CTCGC in the BZR1 promoter on gel-shift and chromatin immunoprecipitationassay and repressed its transcriptionon transienttransformation assay. LIC directly regulated target genessuchasINCREASEDLEAFINCLINATION1(ILI1)toopposetheactionofBZR1.RepressionofLICinILI1transcriptionin protoplastswaspartiallyrescuedbyBZR1.PhenotypicanalysisofthecrossedlinesdepletedinbothLICandBZR1suggested thatBZR1functionallydependsonLIC.MolecularandphysiologyassaysrevealedthatL