Ductal Injection Does Not Increase the Islet Yield or Function after Cold Storage in a Vascular Perfusion Model 英文参考文献.docVIP

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Ductal Injection Does Not Increase the Islet Yield or Function after Cold Storage in a Vascular Perfusion Model 英文参考文献.doc

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Ductal Injection Does Not Increase the Islet Yield or Function after Cold Storage in a Vascular Perfusion Model 英文参考文献

DuctalInjectionDoesNotIncreasetheIsletYieldor FunctionafterColdStorageinaVascularPerfusion Model WataruNakanishi1,TakehiroImura2,AkikoInagaki2,YasuhiroNakamura3,SatoshiSekiguchi1, KeiseiFujimori1,SusumuSatomi1,MasafumiGoto1,2* 1DivisionofAdvancedSurgicalScienceandTechnology,TohokuUniversity,Sendai,Japan,2NewIndustryCreationHatcheryCenter,TohokuUniversity,Sendai,Japan, 3DepartmentofPathology,TohokuUniversitySchoolofMedicine,Sendai,Japan Abstract Severalstudieshavereportedthatpancreaticductalpreservationgreatlyimprovedtheisletyieldandfunctionaftercold storage. However, these studies were devoid of appropriate controls, such as vascular perfusion, which is routinely performedtopreserveorgansintheclinicalsetting.Inthisstudy,wecreatedavascularperfusionmodelusinginbredrats, andinvestigatedtheeffectofductalinjectionontheisletyieldandfunctionaftercoldstorage.Ratpancreasesafter10h coldischemiawereclassifiedasfollows:withoutductal/vascular perfusion;withductalinjection;withvascularperfusion; andwithductal/vascularperfusion.Theisletyield,function,viability,releaseofinflammatorymediators,andpathological changesintheexocrinetissueswereassessedintheHanks’BalancedSaltSolution(HBSS)model.Theisletyieldwasalso assesed by introducing University of Wisconsin Solution (UWS) and Histidine-Tryptophan-Ketoglutarate solution (HTK), which are the standard clinical preservation solutions. In the HBSS model, ductal injection and vascular perfusion significantly improved the islet yield compared with the control group. However, ductal injection showed no additional effectsontheisletyield,function,viabilityandsuppressingthereleaseofinflammatorymediatorswhenvascularperfusion was performed. Although ductal injection significantly decreased the apoptosis of exocrine cells, no beneficial effect on vacuolation was observed. In contrast, vascular perfusion significantly suppressed vacuolation in the exocrine tissues. Likewise,intheUWSandHTKmodel,ductalinjectionandvascularperfusionimprovedtheisle