Duffy Negative Antigen Is No Longer a Barrier to Plasmodium vivax – Molecular Evidences from the African West Coast (Angola and Equatorial Guinea) 英文参考文献.docVIP

下载本文档

6
0
约3.28万字
约 6页
2017-05-11 发布于上海
举报
版权申诉

Duffy Negative Antigen Is No Longer a Barrier to Plasmodium vivax – Molecular Evidences from the African West Coast (Angola and Equatorial Guinea) 英文参考文献.doc

1、本文档共6页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Duffy Negative Antigen Is No Longer a Barrier to Plasmodium vivax – Molecular Evidences from the African West Coast (Angola and Equatorial Guinea) 英文参考文献

DuffyNegativeAntigenIsNoLongeraBarrierto Plasmodiumvivax–MolecularEvidencesfromthe AfricanWestCoast(AngolaandEquatorialGuinea) CristinaMendes1,FernandaDias1,2,JoanaFigueiredo1,VicentaGonzalezMora3,JorgeCano3,Brunode Sousa4,Virg?′lioE.doRosa′rio1,AgustinBenito3,PedroBerzosa3,AnaPaulaArez1* 1Centro de Mala′ria e outras Doenc?as Tropicais, Unidade de Parasitologia, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisbon, Portugal, 2DepartamentodeMedicinaInterna,FaculdadedeMedicina,UniversidadeAgostinhoNeto,Luanda,Angola,3CentroNacionaldeMedicinaTropical,InstitutodeSalud CarlosIII,Madrid,Spain,4CentrodeMala′riaeoutrasDoenc?asTropicais,UnidadedeSau′deInternacional,InstitutodeHigieneeMedicinaTropical,UniversidadeNovade Lisboa,Lisbon,Portugal Abstract Background:PlasmodiumvivaxshowsasmallprevalenceinWestandCentralAfricaduetothehighprevalenceofDuffy negativepeople.However,DuffynegativeindividualsinfectedwithP.vivaxhavebeenreportedinareasofhighprevalence of Duffy positive people who may serve as supply of P. vivax strains able to invade Duffy negative erythrocytes. We investigatedthepresenceofP.vivaxintwoWestAfricancountries,usingbloodsamplesandmosquitoescollectedduring twoon-goingstudies. Methodology/Findings: Blood samples from a total of 995 individuals were collected in seven villages in Angola and Equatorial Guinea, and 820 Anopheles mosquitoes were collected in Equatorial Guinea. Identification of the Plasmodium specieswasachievedbynestedPCRamplificationofthesmall-subunitrRNAgenes;P.vivaxwasfurthercharacterizedbycsp geneanalysis.PositiveP.vivax-humanisolatesweregenotypedfortheDuffybloodgroupthroughtheanalysisoftheDARC gene.FifteenDuffy-negativeindividuals,8fromEquatorialGuinea(outof97)and7fromAngola(outof898),wereinfected withtwodifferentstrainsofP.vivax(VK210andVK247). Conclusions: InthisstudywedemonstratedthatP.vivaxinfectionswerefoundbothinhumansandmosquitoes,which meansthatactivetransmissionisoccurring.Giventhehighprevalenceofinfectioninmos