Dysregulation of MicroRNA-34a Expression in Head and Neck Squamous Cell Carcinoma Promotes Tumor Growth and Tumor Angiogenesis 英文参考文献.docVIP

下载本文档

1
0
约5.51万字
约 13页
2017-05-11 发布于上海
举报
版权申诉

Dysregulation of MicroRNA-34a Expression in Head and Neck Squamous Cell Carcinoma Promotes Tumor Growth and Tumor Angiogenesis 英文参考文献.doc

1、本文档共13页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Dysregulation of MicroRNA-34a Expression in Head and Neck Squamous Cell Carcinoma Promotes Tumor Growth and Tumor Angiogenesis 英文参考文献

DysregulationofMicroRNA-34aExpressioninHeadand NeckSquamousCellCarcinomaPromotesTumorGrowth andTumorAngiogenesis BhavnaKumar1,2,ArtiYadav2,JamesLang1,2,TheodorosN.Teknos1,2,PawanKumar1,2 * 1Department of Otolaryngology-Head and Neck Surgery, The Ohio State University, Columbus, Ohio, United States of America, 2The Ohio State University ComprehensiveCancerCenter,TheOhioStateUniversity,Columbus,Ohio,UnitedStatesofAmerica Abstract Background:MicroRNAs(miRs)aresmallnon-codingRNAsthatplayanimportantroleincancerdevelopmentwherethey canactasoncogenesorastumor-suppressors.miR-34aisatumor-suppressorthatisfrequentlydownregulatedinanumber oftumortypes.However,littleisknownabouttheroleofmiR-34ainheadandnecksquamouscellcarcinoma(HNSCC). MethodsandResults:miR-34aexpressionintumorsamples,HNSCCcelllinesandendothelialcellswasexaminedbyreal time PCR. Lipofectamine-2000 was used to transfect miR-34a in HNSCC cell lines and human endothelial cells. Cell- proliferation, migration and clonogenic survival was examined by MTT, Xcelligence system, scratch assay and colony formationassay.miR-34aeffectontumorgrowthandtumorangiogenesiswasexaminedbyinvivoSCIDmousexenograft model. Our results demonstrate that miR-34a is significantly downregulated in HNSCC tumors and cell lines. Ectopic expressionofmiR-34ainHNSCCcelllinessignificantlyinhibitedtumorcellproliferation,colonyformationandmigration. miR-34a overexpression also markedly downregulated E2F3 and survivin levels. Rescue experiments using microRNA resistant E2F3 isoforms suggest that miR-34a-mediated inhibition of cell proliferation and colony formation is predominantly mediated by E2F3a isoform. In addition, tumor samples from HNSCC patients showed an inverse relationshipbetweenmiR-34aandsurvivinaswellasmiR-34aandE2F3levels.OverexpressionofE2F3acompletelyrescued survivinexpressioninmiR-34aexpressingcells,therebysuggestingthatmiR-34amayberegulatingsurvivinexpressionvia E2F3a.EctopicexpressionofmiR-34aalsosignificantlyinhibitedtumorgrowt