Dysregulation of Human β-Defensin-2 Protein in Inflammatory Bowel Disease 英文参考文献.docVIP

下载本文档

6
0
约4.88万字
约 11页
2017-05-11 发布于上海
举报
版权申诉

Dysregulation of Human β-Defensin-2 Protein in Inflammatory Bowel Disease 英文参考文献.doc

1、本文档共11页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Dysregulation of Human β-Defensin-2 Protein in Inflammatory Bowel Disease 英文参考文献

DysregulationofHumanb-Defensin-2Proteinin InflammatoryBowelDisease MarianC.Aldhous*,ColinL.Noble,JackSatsangi Gastrointestinal Unit, School of Clinical and Molecular Medicine, University of Edinburgh, Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh,Scotland,UnitedKingdom Abstract Background:Humanb-defensin-2(HBD2)isanantimicrobialpeptideimplicatedinthepathogenesisofinflammatorybowel disease (IBD). Low copy number and concomitant low mRNA expression of the HBD2 gene have been implicated in susceptibilitytocolonicCrohn’sDisease(CD).WeinvestigatedthecolonicdistributionofHBD2mRNAexpression,andthe contributionsofgeneticandenvironmentalfactorsonHBD2proteinproduction. Methodology/PrincipalFindings:WeexaminedHBD2mRNAexpressionatthreecoloniclocationsbymicroarrayanalysisof biopsies from 151 patients (53 CD, 67 ulcerative colitis [UC], 31 controls). We investigated environmental and genetic influencesonHBD2proteinproductionusingexvivoculturedsigmoidcolonbiopsiesfrom69patients(22CD,26UC,21 controls) stimulated with lipopolysaccharide (LPS) and/or nicotine for 24hours. HBD2 and cytokines were measured in culturesupernatants.UsingDNAsamplesfromthesepatients,regionsintheHBD2genepromoterweresequencedforNF- kB binding-sites and HBD2 gene copy number was determined. HBD2 mRNA expression was highest in inflamed (vs. uninflamed p=0.0122) ascending colon in CD and in inflamed (vs. uninflamed p,0.0001) sigmoid colon in UC. HBD2 proteinproductionwasincreasedininflamedUCbiopsies(p=0.0078).TherewasnodifferenceinHBD2proteinproduction from unstimulated biopsies of CD, UC and controls. LPS-induced HBD2 production was significantly increased in CD (p=0.0375)butnotUC(p=0.2017);thisLPS-inducedresponsewasaugmentedbynicotineinUC(p=0.0308)butnotCD (p=0.6872). Nicotine alone did not affect HBD2 production. HBD2 production correlated with IL8 production in UC (p,0.001)andwithIL10inCD(p,0.05).VariationsintheHBD2promoterandHBD2genecopynumberdidnotaffectHBD2 production