Effects and Action Mechanisms of Berberine and Rhizoma coptidis on Gut Microbes and Obesity in High-Fat Diet-Fed C57BL6J Mice 英文参考文献.docVIP

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Effects and Action Mechanisms of Berberine and Rhizoma coptidis on Gut Microbes and Obesity in High-Fat Diet-Fed C57BL6J Mice 英文参考文献.doc

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Effects and Action Mechanisms of Berberine and Rhizoma coptidis on Gut Microbes and Obesity in High-Fat Diet-Fed C57BL6J Mice 英文参考文献

EffectsandActionMechanismsofBerberineand RhizomacoptidisonGutMicrobesandObesityin High-FatDiet-FedC57BL/6JMice WeidongXie1.,DayongGu2.,JiannaLi1,KaiCui1,YaouZhang1* 1LifeScienceDivision,GraduateSchoolatShenzhen,TsinghuaUniversity,Shenzhen,China,2InstituteofDiseaseControlandPrevention,ShenzhenInternationalTravel HealthCareCenter,ShenzhenEntry-ExitInspectionandQuarantineBureau,Shenzhen,China Abstract Gut microbes play important roles in regulating fat storage and metabolism. Rhizoma coptidis (RC) and its main active compound, berberine, have either antimicrobial or anti-obesity activities. In the present study, we hypothesize that RC exertsanti-obesityeffectsthatarelikelymediatedbymechanismsofregulatinggutmicrobesandberberinemaybeakey compound of RC. Gut microbes and glucose and lipid metabolism in high-fat diet-fed C57BL/6J (HFD) mice in vivo are investigated after RC and berberine treatments. The results show that RC (200mg/kg) and berberine (200mg/kg) significantly lower both body and visceral adipose weights, and reduce blood glucose and lipid levels, and decrease degradation ofdietary polysaccharides inHFDmice.BothRCandberberinesignificantlyreducetheproportionsoffecal FirmicutesandBacteroidetestototalbacteriainHFDmice.Inthetrialexvivo,bothRCandberberinesignificantlyinhibit thegrowthofgutbacteriaunderaerobicandanaerobicconditions.Ininvitrotrials,bothRCandberberinesignificantly inhibitthegrowthofLactobacillus (aclassical typeofFirmicutes)underanaerobicconditions.Furthermore,bothRCand berberine significantly increase fasting-induced adipose factor (Fiaf, a key protein negatively regulated by intestinal microbes) expressions in either intestinal or visceral adipose tissues. Both RC and berberine significantly increase mRNA expressionsofAMPK,PGC1a,UCP2,CPT1a,andHadhbrelatedtomitochondrialenergymetabolism,whichmaybedriven by increased Fiaf expression. These results firstly suggest that antimicrobial activities of RC and berberine may result in decreasingdegradation