eIF5A Promotes Translation Elongation, Polysome Disassembly and Stress Granule Assembly 英文参考文献.docVIP

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eIF5A Promotes Translation Elongation, Polysome Disassembly and Stress Granule Assembly 英文参考文献.doc

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eIF5A Promotes Translation Elongation, Polysome Disassembly and Stress Granule Assembly 英文参考文献

eIF5APromotesTranslationElongation,Polysome DisassemblyandStressGranuleAssembly ChiHoLi.,TakbumOhn.¤,PavelIvanov,SarahTisdale,PaulAnderson* DivisionofRheumatology,ImmunologyandAllergy,BrighamandWomen’sHospital,Boston,Massachusetts,UnitedStatesofAmerica Abstract Stress granules (SGs) are cytoplasmic foci at which untranslated mRNAs accumulate in cells exposed to environmental stress. We have identified ornithine decarboxylase (ODC), an enzyme required for polyamine synthesis, and eIF5A, a polyamine (hypusine)-modified translation factor, as proteins required for arsenite-induced SG assembly. Knockdown of deoxyhypusinesynthase(DHS)ortreatmentwithadeoxyhypusinesynthaseinhibitor(GC7)preventshypusinemodification ofeIF5Aaswellasarsenite-inducedpolysomedisassemblyandstressgranuleassembly.Time-courseanalysisrevealsthat thisisduetoaslowingofstress-inducedribosomerun-offincellslackinghypusine-eIF5A.WhereaseIF5Aonlymarginally affects protein synthesis under normal conditions, it is required for the rapid onset of stress-induced translational repression. Our results reveal that hypusine-eIF5A-facilitated translation elongation promotes arsenite-induced polysome disassemblyandstressgranuleassemblyincellssubjectedtoadverseenvironmentalconditions. Citation:LiCH,OhnT,IvanovP,TisdaleS,AndersonP(2010)eIF5APromotesTranslationElongation,PolysomeDisassemblyandStressGranuleAssembly.PLoS ONE5(4):e9942.doi:10.1371/journal.pone.0009942 Editor:MattKaeberlein,UniversityofWashington,UnitedStatesofAmerica ReceivedJanuary21,2010;AcceptedMarch9,2010;PublishedApril1,2010 Copyright:?2010Lietal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwassupportedbyNationalInstitutesofHealth(NIH)grantsAI065858,AI033600andAR0514732.Thefundershadnoroleinstudydesign, datacollectionandanalysis,decisiontopublish,orpreparationofthemanuscript.