Effects of the Insemination of Hydrogen Peroxide-Treated Epididymal Mouse Spermatozoa on γH2AX Repair and Embryo Development 英文参考文献.docVIP

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Effects of the Insemination of Hydrogen Peroxide-Treated Epididymal Mouse Spermatozoa on γH2AX Repair and Embryo Development 英文参考文献.doc

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Effects of the Insemination of Hydrogen Peroxide-Treated Epididymal Mouse Spermatozoa on γH2AX Repair and Embryo Development 英文参考文献

EffectsoftheInseminationofHydrogenPeroxide- TreatedEpididymalMouseSpermatozoaoncH2AX RepairandEmbryoDevelopment JianfengXiao1.,YanmeiLiu2.,ZhilingLi1*,YongcuiZhou1,HongLin1,XiaoyanWu1,ManChen1, WanfenXiao1 1Reproductive Center, The First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou, Guangdong, People’s Republic of China, 2ReproductiveCenter,WomenandChildrenHealthcareHospital,Qinzhou,Guangxi,People’sRepublicofChina Abstract Background:Cryopreservationofhumansemenforassistedreproductioniscomplicatedbycryodamagetospermatozoa causedbyexcessivereactiveoxygenspecies(ROS)generation. MethodsandFindings:WeusedexogenousROS(H2O2)tosimulatecryopreservationandexaminedDNAdamagerepairin embryosfertilizedwithspermwithH2O2-inducedDNAdamage.Spermsampleswerecollectedfromepididymisofadult maleKMmiceandtreatedwithcapacitationmedium(containing0,0.1,0.5and1mMH2O2)orcryopreservation.Themodel of DNA-damaged sperm was based on sperm motility, viability and the expression of cH2AX, the DNA damage-repair marker.Weexaminedfertilityrate,development,cellcleavage,andcH2AXlevelinembryosfertilizedwithDNA-damaged sperm.Cryopreservationand1-mMH2O2treatmentproducedsimilarDNAdamage.Mostoftheone-andtwo-cellembryos fertilized with DNA-damaged sperm showed a delay in cleavage before the blastocyst stage. Immunocytochemistry revealedcH2AXintheone-andfour-cellembryos. Conclusions:cH2AXmaybeinvolvedinrepairofpreimplantationembryosfertilizedwithoxygen-stressedspermatozoa. Citation:XiaoJ,LiuY,LiZ,ZhouY,LinH,etal.(2012)EffectsoftheInseminationofHydrogenPeroxide-TreatedEpididymalMouseSpermatozoaon cH2AX RepairandEmbryoDevelopment.PLoSONE7(6):e38742.doi:10.1371/journal.pone.0038742 Editor:JohnR.Battista,LouisianaStateUniversityandAMCollege,UnitedStatesofAmerica ReceivedMarch9,2012;AcceptedMay10,2012;PublishedJune26,2012 Copyright:?2012Xiaoetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted us