Endoreplication Controls Cell Fate Maintenance 英文参考文献.docVIP

EndoreplicationControlsCellFateMaintenance JonathanBramsiepe1,KatjaWester2¤a,ChristinaWeinl3¤b,FarshadRoodbarkelari3¤c,RemmyKasili4¤d , JohnC.Larkin4,MartinHu¨lskamp2,ArpSchnittger1,3* 1Institut de Biologie Mole′culaire des Plantes du CNRS, Universite′ de Strasbourg, Strasbourg, France, 2Lehrstuhl fu¨r Botanik III, Universita¨t zu Ko¨ln, Ko¨ln, Germany, 3UnigruppeamMax-Planck-Institutfu¨rPflanzenzu¨chtungsforschung,Lehrstuhlfu¨rBotanikIII,Universita¨t zuKo¨ln,Ko¨ln,Germany,4DepartmentofBiologicalSciences, LouisianaStateUniversity,BatonRouge,Louisiana,UnitedStatesofAmerica Abstract Cell-fate specification is typically thought to precede and determine cell-cycle regulation during differentiation. Here we show that endoreplication, also known as endoreduplication, a specialized cell-cycle variant often associated with cell differentiationbutalsofrequentlyoccurringinmalignantcells,playsaroleinmaintainingcellfate.Forourstudywehave used Arabidopsis trichomes as a model system and have manipulated endoreplication levels via mutants of cell-cycle regulators and overexpression of cell-cycle inhibitors under a trichome-specific promoter. Strikingly, a reduction of endoreplication resulted in reduced trichome numbers and caused trichomes to lose their identity. Live observations of young Arabidopsis leaves revealed that dedifferentiating trichomes re-entered mitosis and were re-integrated into the epidermalpavement-celllayer,acquiringthetypicalcharacteristicsofthesurroundingepidermalcells.Conversely,whenwe promoted endoreplication in glabrous patterning mutants, trichome fate could be restored, demonstrating that endoreplication is an important determinant of cell identity. Our data lead to a new model of cell-fate control and tissueintegrityduringdevelopmentbyrevealingacell-fatequalitycontrolsystematthetissuelevel. Citation: Bramsiepe J, Wester K, Weinl C, Roodbarkelari F, Kasili R, et al. (2010) Endoreplication Controls Cell Fate Maintenance. PLoS Genet 6(6): e100099