Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory Pathogens 英文参考文献.docVIP

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Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory Pathogens 英文参考文献.doc

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Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory Pathogens 英文参考文献

EquivalenceofSelf-andStaff-CollectedNasalSwabsfor theDetectionofViralRespiratoryPathogens ManasK.Akmatov1*,AnjaGatzemeier1,KlausSchughart2,3,FrankPessler1,4 1Department of Epidemiology, HelmholtzCentrefor InfectionResearch,Braunschweig, Germany, 2Department of Infection Genetics, HelmholtzCentre for Infection Research,Braunschweig,Germany,3UniversityofVeterinaryMedicineHannover,Hannover,Germany,4DivisionofRheumatologyandImmunology,UniversityChildren’s Hospital,TechnicalUniversityDresden,Dresden,Germany Abstract Background:Theneedforthetimelycollectionofdiagnosticbiosamplesduringsymptomaticepisodesrepresentsamajor obstacletolarge-scalestudiesonacuterespiratoryinfection(ARI)epidemiology.Thismaybecircumventedbyhavingthe participantscollecttheirownnasalswabs.Wecomparedself-andstaff-collectedswabsintermsofswabbingqualityand detectionofviralrespiratorypathogens. Methodology/PrincipalFindings:WeconductedaprospectivestudyamongemployeesofourinstitutionduringtheARI season2010/2011(December-March).Weeklyemailsweresenttotheparticipants(n=84),remindingthemtocometothe studycenterincaseofnewsymptoms.Theparticipantsself-collectedananteriornasalswabfromonenostril,andtrained study personnel collected one from the other nostril. The participants self-collected another two swabs (one from each nostril) onasubsequentday. Humanb-actinDNAconcentration wasdeterminedintheswabsas aqualitycontrol. Viral respiratory pathogens were detected by multiplex RT-PCR (Seeplex RV15 kit, Seegene, Eschborn, Germany). Of 84 participants,56(67%)reportedatleastoneARIepisode,18participantstwo,andoneparticipantthree.Self-swabbingwas highlyacceptedbytheparticipants.Theamountofb-actinDNAperswabwashigherintheself-thaninthestaff-collected swabs (p=0.008). b-actin concentration was lower in the self-swabs collected on day 1 than in those collected on a subsequentday(p,0.0001).Arespiratoryviralpathogenwasdetectedin31%(23/75)ofstaff-andin35%(26/75)ofself- collectedswabs(p=0.36).Withbothapproaches,themostfre