Ethylene Receptors Function as Components of High-Molecular-Mass Protein Complexes in Arabidopsis 英文参考文献.docVIP

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Ethylene Receptors Function as Components of High-Molecular-Mass Protein Complexes in Arabidopsis 英文参考文献.doc

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Ethylene Receptors Function as Components of High-Molecular-Mass Protein Complexes in Arabidopsis 英文参考文献

EthyleneReceptorsFunctionasComponentsofHigh- Molecular-MassProteinComplexesinArabidopsis Yi-FengChen1.¤a,ZhiyongGao1.¤b,RobertJ.KerrisIII1.,WuyiWang2¤c,BradM.Binder2¤d,G.Eric Schaller1* 1DepartmentofBiologicalSciences,DartmouthCollege,Hanover,NewHampshire,UnitedStatesofAmerica,2DepartmentofBotany,UniversityofWisconsin,Madison, Wisconsin,UnitedStatesofAmerica Abstract Background:ThegaseousplanthormoneethyleneisperceivedinArabidopsisthalianabyafive-memberreceptorfamily composedofETR1,ERS1,ETR2,ERS2,andEIN4. Methodology/Principal Findings: Gel-filtration analysis of ethylene receptors solubilized from Arabidopsis membranes demonstratesthatthereceptorsexistascomponentsofhigh-molecular-massproteincomplexes.TheERS1proteincomplex exhibits an ethylene-induced change in size consistent with ligand-mediated nucleation of protein-protein interactions. DeletionanalysissupportstheparticipationofmultipledomainsfromETR1informationoftheproteincomplex,andalso demonstratesthattargetingtoandretentionofETR1attheendoplasmicreticulumonlyrequiresthefirst147aminoacids ofthereceptor.ArolefordisulfidebondsinstabilizingtheETR1proteincomplexwasdemonstratedbyuseofreducing agentsandmutationofCys4andCys6ofETR1.ExpressionandanalysisofETR1inatransgenicyeastsystemdemonstrates theimportanceofCys4andCys6ofETR1instabilizingthereceptorforethylenebinding. Conclusions/Significance: These data support the participation of ethylene receptors in obligate as well as ligand- dependent non-obligate protein interactions. These data also suggest that different protein complexes may allow for tailoringoftheethylenesignaltospecificcellularenvironmentsandresponses. Citation:ChenY-F,GaoZ,KerrisRJIII,WangW,BinderBM,etal.(2010)EthyleneReceptorsFunctionasComponentsofHigh-Molecular-MassProteinComplexes inArabidopsis.PLoSONE5(1):e8640.doi:10.1371/journal.pone.0008640 Editor:KarinSchumacher,UniversityofHeidelberg,Germany ReceivedNovember15,2009;AcceptedDecember18,2009;PublishedJanuary8,2010 Copyright: ? 2010 Chen et al.