Gene Families of Cuticular Proteins Analogous to Peritrophins (CPAPs) in Tribolium castaneum Have Diverse Functions 英文参考文献.docVIP

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Gene Families of Cuticular Proteins Analogous to Peritrophins (CPAPs) in Tribolium castaneum Have Diverse Functions 英文参考文献.doc

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Gene Families of Cuticular Proteins Analogous to Peritrophins (CPAPs) in Tribolium castaneum Have Diverse Functions 英文参考文献

GeneFamiliesofCuticularProteinsAnalogousto Peritrophins(CPAPs)inTriboliumcastaneumHave DiverseFunctions SinuJasrapuria1,CharlesA.Specht2,KarlJ.Kramer1,3,RichardW.Beeman3, SubbaratnamMuthukrishnan1* 1DepartmentofBiochemistry,KansasStateUniversity,Manhattan,Kansas,UnitedStatesofAmerica,2DepartmentofMedicine,UniversityofMassachusetts,Worcester, Massachusetts,UnitedStatesofAmerica,3USDA-ARSCenterforGrainandAnimalHealthResearchCenter,Manhattan,Kansas,UnitedStatesofAmerica Abstract Thefunctionalcharacterizationofanentireclassof17genesfromtheredflourbeetle,Triboliumcastaneum,whichencode twofamiliesofCuticularProteinsAnalogoustoPeritrophins(CPAPs)hasbeencarriedout.CPAPgenesinT.castaneumare expressed exclusively in cuticle-forming tissues and have been classified into two families, CPAP1 and CPAP3, based on whethertheproteinscontaineitherone(CPAP1),orthreecopies(CPAP3)ofthechitin-bindingdomain,ChtBD2,withitssix characteristically spaced cysteine residues. Individual members of the TcCPAP1 and TcCPAP3 gene families have distinct developmentalpatternsofexpression.Manyoftheseproteinsserveessentialandnon-redundantfunctionsinmaintaining thestructuralintegrityofthecuticleindifferentpartsoftheinsectanatomy.ThreegenesoftheTcCPAP1familyandfive genesoftheTcCPAP3familyareessentialforinsectdevelopment,molting,cuticleintegrity,properlocomotionorfecundity. RNA interference (RNAi) targeting TcCPAP1-C, TcCPAP1-H, TcCPAP1-J or TcCPAP3-C transcripts resulted in death at the pharate adult stage of development. RNAi for TcCPAP3-A1, TcCPAP3-B, TcCPAP3-D1 or TcCPAP3-D2 genes resulted in differentdevelopmentaldefects,includingadult/embryonicmortality,abnormalelytraorhindwings,oranabnormal‘stiff- jointed’gait.TheseresultsprovideexperimentalsupportforspecializationinthefunctionsofCPAPproteinsinT.castaneum andabiologicalrationalefortheconservationofCPAPorthologsinotherordersofinsects.Thisisthefirstcomprehensive functionalanalysisofanentireclassofcuticularproteinswithoneormoreChtBD2domainsinanyinsec