Genetic and Physical Mapping of Candidate Genes for Resistance to Fusarium oxysporum f.sp. tracheiphilum Race 3 in Cowpea [Vigna unguiculata (L.) Walp] 英文参考文献.docVIP

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Genetic and Physical Mapping of Candidate Genes for Resistance to Fusarium oxysporum f.sp. tracheiphilum Race 3 in Cowpea [Vigna unguiculata (L.) Walp] 英文参考文献.doc

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Genetic and Physical Mapping of Candidate Genes for Resistance to Fusarium oxysporum f.sp. tracheiphilum Race 3 in Cowpea [Vigna unguiculata (L.) Walp] 英文参考文献

GeneticandPhysicalMappingofCandidateGenesfor ResistancetoFusariumoxysporumf.sp.tracheiphilum Race3inCowpea[Vignaunguiculata(L.)Walp] MartiPottorff1,SteveWanamaker1,YaqinQ.Ma1,JeffreyD.Ehlers1,2,PhilipA.Roberts3, TimothyJ.Close1* 1Department of Botany Plant Sciences, University of California Riverside, Riverside California, United States of America, 2Bill Melinda Gates Foundation, Seattle, Washington,UnitedStatesofAmerica,3DepartmentofNematology,UniversityofCaliforniaRiverside,Riverside,California,UnitedStatesofAmerica Abstract Fusariumoxysporumf.sp.tracheiphilum(Fot)isasoil-bornefungalpathogenthatcausesvascularwiltdiseaseincowpea.Fot race 3 is one of the major pathogens affecting cowpea production in California. Identification of Fot race 3 resistance determinantswillexpeditedeliveryofimprovedcultivarsbyreplacingtime-consumingphenotypicscreeningwithselection based on perfect markers, thereby generating successful cultivars in a shorter time period. Resistance to Fot race 3 was studiedintheRILpopulationCaliforniaBlackeye27(resistant)x24-125B-1(susceptible).BiparentalmappingidentifiedaFot race 3 resistance locus, Fot3-1, which spanned 3.56cM on linkage group one of the CB27 x 24-125B-1 genetic map. A marker-trait association narrowed the resistance locus to a 1.2cM region and identified SNP marker 1_1107 as co- segregatingwithFot3-1resistance.MacroandmicrosyntenywasobservedfortheFot3-1locusregioninGlycinemaxwhere six disease resistance genes were observed in the two syntenic regions of soybean chromosomes 9 and 15. Fot3-1 was identifiedonthecowpeaphysicalmaponBACcloneCH093L18,spanningapproximately208,868bponBACcontig250. TheFot3-1locuswasnarrowedto0.5cMdistanceonthecowpeageneticmaplinkagegroup6,flankedbySNPmarkers 1_0860and1_1107.BACcloneCH093L18wassequencedandfourcowpeasequenceswithsimilaritytoleucine-richrepeat serine/threonineproteinkinaseswereidentifiedandarecowpeacandidategenesfortheFot3-1locus.Thisstudyhasshown howreadilycandidategenescanbeidentifiedforsimp