Genetic Variation within Native Populations of Endemic Silkmoth Antheraea assamensis (Helfer) from Northeast India Indicates Need for In Situ Conservation 英文参考文献.docVIP

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Genetic Variation within Native Populations of Endemic Silkmoth Antheraea assamensis (Helfer) from Northeast India Indicates Need for In Situ Conservation 英文参考文献.doc

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Genetic Variation within Native Populations of Endemic Silkmoth Antheraea assamensis (Helfer) from Northeast India Indicates Need for In Situ Conservation 英文参考文献

GeneticVariationwithinNativePopulationsofEndemic SilkmothAntheraeaassamensis(Helfer)fromNortheast IndiaIndicatesNeedforInSituConservation Y.TunginbaSingh1,3,SudeshnaMazumdar-Leighton1*,MahaswettaSaikia1,PrashantPant1, SochanngamKashung1,KartikNeog2,RajenChakravorty2,SureshNair3,JavaregowdaNagaraju4, CheerukeriRaghavendraBabu5 1Department of Botany, University of Delhi, Delhi, India, 2Central Muga and Eri Research and Training Institute, Ladoigarh, Assam, India, 3International Centre for GeneticEngineeringBiotechnology,NewDelhi,India,4CenterforDNAFingerprintingDiagnostics,Hyderabad,AndhraPradesh,India,5CenterforManagementof DegradedEcosystems,UniversityofDelhi,Delhi,India Abstract A. assamensis is a phytophagous Lepidoptera from Northeast India reared on host trees of Lauraceae family for its characteristiccocoonsilk.Sourceofthesecocoonsaredomesticatedfarmstocksthatcrashfrequentlyand/orwildinsect populations that provide new cultures. The need to reduce dependence on wild populations for cocoons necessitates assessment of genetic diversity in cultivated and wild populations. Molecular markers based on PCR of Inter-simple sequence repeats (ISSR) and simple sequence repeats (SSR) were used with four populations of wild insects and eleven populationsofcultivatedinsects.Wildpopulationshadhighgeneticdiversityestimates(Hi=0.25;HS=0.28;HE=0.42)and atleastonepopulationcontainedprivatealleles.Bothmarkersystemsindicatedthatgeneticvariabilitywithinpopulations examined was significantly high. Among cultivated populations, insects of the Upper Assam region (Hi=0.19; HS =0.18; HE=0) were genetically distinct (FST=0.38 with both marker systems) from insects of Lower Assam (Hi=0.24; HS =0.25; HE=0.3).Sequencingofpolymorphicampliconssuggestedtranspositionasamechanismformaintaininggenomicdiversity. Implicationsforconservationofnativepopulationsinthewildandpreservingin-farmdiversityarediscussed. Citation:SinghYT,Mazumdar-LeightonS,SaikiaM,PantP,KashungS,etal.(2012)GeneticVariationwit