Genome-Wide Analyses of Gene Expression during Mouse Endochondral Ossification 英文参考文献.docVIP

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Genome-Wide Analyses of Gene Expression during Mouse Endochondral Ossification 英文参考文献.doc

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Genome-Wide Analyses of Gene Expression during Mouse Endochondral Ossification 英文参考文献

Genome-WideAnalysesofGeneExpressionduring MouseEndochondralOssification ClaudineG.James1,Lee-AnneStanton1,HangaAgoston1,VeronicaUlici1*,T.MichaelUnderhill2 ,Frank Beier1* 1CIHRGroupinSkeletalDevelopmentandRemodelling,DepartmentofPhysiologyandPharmacology,SchulichSchoolofMedicineandDentistry,UniversityofWestern Ontario,London,Canada,2DepartmentofCellularandPhysiologicalSciences,UniversityofBritishColumbia,Vancouver,BritishColumbia,Canada Abstract Background: Endochondral ossification is a complex process involving a series of events that are initiated by the establishmentofachondrogenictemplateandculminateinitsreplacementthroughthecoordinatedactivityofosteoblasts, osteoclasts andendothelialcells. Comprehensive analysesof invivo geneexpressionprofiles during theseprocesses are essentialtoobtainacompleteunderstandingoftheregulatorymechanismsinvolved. Methodology/PrincipalFindings:Toaddresstheseissues,wecompletedamicroarrayscreenofthreezonesderivedfrom manually segmented embryonic mouse tibiae. Classification of genes differentially expressed between each respective zone,functionalcategorizationaswellascharacterizationofgeneexpressionpatterns,cytogeneticloci,signalingpathways and functional motifs both confirmed reported data and provided novel insights into endochondral ossification. Parallel comparisons of the microdissected tibiae data set with our previously completed micromass culture screen further corroborated the suitability of micromass cultures for modeling gene expression in chondrocyte development. The micromass culture system demonstrated striking similarities to the in vivo microdissected tibiae screen; however, the micromasssystemwasunabletoaccuratelydistinguishgeneexpressiondifferencesinthehypertrophicandmineralized zonesofthetibia. Conclusions/Significance:Thesestudiesallowustobetterunderstandgeneexpressionpatternsinthegrowthplateand endochondral bones and provide an important technical resource for comparison of gene expression in diseased or experime