Grape Proanthocyanidin Inhibit Pancreatic Cancer Cell Growth In Vitro and In Vivo through Induction of Apoptosis and by Targeting the PI3KAkt Pathway 英文参考文献.docVIP

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Grape Proanthocyanidin Inhibit Pancreatic Cancer Cell Growth In Vitro and In Vivo through Induction of Apoptosis and by Targeting the PI3KAkt Pathway 英文参考文献.doc

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Grape Proanthocyanidin Inhibit Pancreatic Cancer Cell Growth In Vitro and In Vivo through Induction of Apoptosis and by Targeting the PI3KAkt Pathway 英文参考文献

GrapeProanthocyanidinInhibitPancreaticCancerCell GrowthInVitroandInVivothroughInductionof ApoptosisandbyTargetingthePI3K/AktPathway RamPrasad2,MuditVaid2,SantoshK.Katiyar1,2,3,4* 1Birmingham VeteransAffairsMedicalCenter,Birmingham,Alabama,UnitedStatesofAmerica, 2DepartmentofDermatology,UniversityofAlabamaatBirmingham, Birmingham, Alabama, United States of America, 3Nutrition Obesity Research Center, University of Alabama at Birmingham, Birmingham, Alabama, United States of America,4ComprehensiveCancerCenter,UniversityofAlabamaatBirmingham,Birmingham,Alabama,UnitedStatesofAmerica Abstract Pancreaticcancerisanaggressivemalignancythatisfrequentlydiagnosedatanadvancedstagewithpoorprognosis.Here, wereportthechemotherapeuticeffectsofbioactiveproanthocyanidinsfromgrapeseeds(GSPs)asassessedusingInVitro andInVivomodels.Treatmentofhumanpancreaticcancercells(Miapaca-2,PANC-1andAsPC-1)withGSPsInVitroreduced cell viability and increased G2/M phase arrest of the cell cycle leading to induction of apoptosis in a dose- and time- dependentmanner.TheGSPs-inducedapoptosisofpancreaticcancercellswasassociatedwithadecreaseinthelevelsof Bcl-2andBcl-xlandanincreaseinthelevelsofBaxandactivatedcaspase-3.TreatmentofMiapaca-2andPANC-1cellswith GSPs also decreased the levels of phosphatidylinositol-3-kinase (PI3K) and phosphorylation of Akt at ser473 . siRNA knockdownofPI3KfrompancreaticcancercellsalsoreducedthephosphorylationofAkt.Further,dietaryadministrationof GSPs(0.5%,w/w)asasupplementedAIN76AcontroldietsignificantlyinhibitedthegrowthofMiapaca-2pancreatictumor xenograftsgrownsubcutaneouslyinathymicnudemice,whichwasassociatedwith:(i)inhibitionofcellproliferation,(ii) inductionofapoptosisoftumorcells,(iii)increasedexpressionofBax,reducedexpressionofanti-apoptoticproteinsand activationofcaspase-3-positivecells,and(iv)decreasedexpressionofPI3Kandp-Aktintumorxenografttissues.Together, theseresultssuggestthatGSPsmayhaveapotentialchemotherapeuticeffectonpancreaticcancercellgrowth. Citation