Microarray Profiling of Phage-Display Selections for Rapid Mapping of Transcription Factor–DNA Interactions 英文参考文献.docVIP

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Microarray Profiling of Phage-Display Selections for Rapid Mapping of Transcription Factor–DNA Interactions 英文参考文献.doc

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Microarray Profiling of Phage-Display Selections for Rapid Mapping of Transcription Factor–DNA Interactions 英文参考文献

MicroarrayProfilingofPhage-DisplaySelectionsfor RapidMappingofTranscriptionFactor–DNAInteractions GordonFreckleton1,2¤,SoyeonI.Lippman1,2,JamesR.Broach1,2,SaeedTavazoie1,2 * 1DepartmentofMolecularBiology,PrincetonUniversity,Princeton,NewJersey,UnitedStatesofAmerica,2TheLewis-SiglerInstituteforIntegrativeGenomics,Princeton University,Princeton,NewJersey,UnitedStatesofAmerica Abstract Modern computational methods are revealing putative transcription-factor (TF) binding sites at an extraordinary rate. However,themajorchallengeinstudyingtranscriptionalnetworksistomaptheseregulatoryelementpredictionstothe proteintranscriptionfactorsthatbindthem.Wehavedevelopedamicroarray-based profiling ofphage-display selection (MaPS)strategythatallowsrapidandglobalsurveyofanorganism’sproteomeforsequence-specificinteractionswithsuch putative DNA regulatory elements. Application to a variety of known yeast TF binding sites successfully identified the cognateTFfromthebackgroundofacomplexwhole-proteomelibrary.ThesefactorscontainDNA-bindingdomainsfrom diverse families, including Myb, TEA, MADS box, and C2H2 zinc-finger. Using MaPS, we identified Dot6 as a trans-active partnerofthelong-predictedorphanyeastelementPolymerase AC(PAC).MaPStechnologyshouldenablerapidand proteome-scalestudyofbi-molecularinteractionswithintranscriptionalnetworks. Citation:FreckletonG,LippmanSI,BroachJR,TavazoieS(2009)MicroarrayProfilingofPhage-DisplaySelectionsforRapidMappingofTranscriptionFactor–DNA Interactions.PLoSGenet5(4):e1000449.doi:10.1371/journal.pgen.1000449 Editor:MichaelSnyder,YaleUniversity,UnitedStatesofAmerica ReceivedOctober19,2008;AcceptedMarch10,2009;PublishedApril10,2009 Copyright: ?2009 Freckleton et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:TheresearchissupportedbygrantsfromtheNSFEarlyCareerDevel