MicroRNA Profiling during Cardiomyocyte-Specific Differentiation of Murine Embryonic Stem Cells Based on Two Different miRNA Array Platforms 英文参考文献.docVIP

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MicroRNA Profiling during Cardiomyocyte-Specific Differentiation of Murine Embryonic Stem Cells Based on Two Different miRNA Array Platforms 英文参考文献.doc

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MicroRNA Profiling during Cardiomyocyte-Specific Differentiation of Murine Embryonic Stem Cells Based on Two Different miRNA Array Platforms 英文参考文献

MicroRNAProfilingduringCardiomyocyte-Specific DifferentiationofMurineEmbryonicStemCellsBasedon TwoDifferentmiRNAArrayPlatforms LinGan1,SilkeSchwengberg2,BerndDenecke1* 1InterdisciplinaryCentreforClinicalResearch(IZKF)Aachen,RWTHAachenUniversity,Aachen,Germany,2AxiogenesisAG,Ko¨ln,Germany Abstract MicroRNA (miRNA) plays a critical role in a wide variety of biological processes. Profiling miRNA expression during differentiationofembryonicstemcellswillhelptounderstandtheregulationpathwayofdifferentiation,whichinturnmay elucidatediseasemechanisms.TheidentifiedmiRNAscouldthenserveasanewgroupofpossibletherapeutictargets.Inthe presentpaper,miRNAexpressionprofilesweredeterminedduringcardiomyocyte-specificdifferentiationandmaturationof murine embryonic stem (ES) cells. For this purpose a homogeneous cardiomyocyte population was generated from a transgenicmurineEScellline.Twohighthroughputarrayplatforms(AffymetrixandFebit)wereusedformiRNAprofilingin order to compare the effect of the platforms on miRNA profiling as well as to increase the validity of target miRNA identification.Four time points(i.e.day0,day12,day19and day26)werechosenforthemiRNAprofilingstudy,which corresponded to different stages during cardiomyocyte-specific differentiation and maturation. Fifty platform and pre- processing method-independent miRNAs were identified as being regulated during the differentiation and maturation processes.TheidentificationofthesemiRNAsisanimportantstepforcharacterizingandunderstandingtheeventsinvolvedin cardiomyocyte-specificdifferentiationofEScellsandmayalsohighlightcandidatetargetmoleculesfortherapeuticpurposes. Citation:GanL,SchwengbergS,DeneckeB(2011)MicroRNAProfilingduringCardiomyocyte-SpecificDifferentiationofMurineEmbryonicStemCellsBasedon TwoDifferentmiRNAArrayPlatforms.PLoSONE6(10):e25809.doi:10.1371/journal.pone.0025809 Editor:MarcTjwa,UniversityofFrankfurt-UniversityHospitalFrankfurt,Germany ReceivedSeptember15,2010;AcceptedSeptember11,2011;PublishedOctober3,20