Multiple Lineages of Human Breast Cancer StemProgenitor Cells Identified by Profiling with Stem Cell Markers 英文参考文献.docVIP

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Multiple Lineages of Human Breast Cancer StemProgenitor Cells Identified by Profiling with Stem Cell Markers 英文参考文献.doc

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Multiple Lineages of Human Breast Cancer StemProgenitor Cells Identified by Profiling with Stem Cell Markers 英文参考文献

MultipleLineagesofHumanBreastCancerStem/ ProgenitorCellsIdentifiedbyProfilingwithStemCell Markers WendyW.Hwang-Verslues1,Wen-HungKuo2,Po-HaoChang1,Chi-ChunPan1,Hsing-HuiWang1 ,Sheng- TaTsai1,Yung-MingJeng3,Jin-YuShew1,JohnT.Kung4,Chung-HsuanChen1,EvaY.-H.P.Lee1,5,6* ,King- JenChang2*,Wen-HwaLee1,6* 1GenomicsResearchCenter,AcademiaSinica,Taipei,Taiwan,2DepartmentofSurgery,NationalTaiwanUniversityHospital,Taipei,Taiwan,3DepartmentofPathology,National TaiwanUniversityHospital,Taipei,Taiwan,4InstituteofMolecularBiology,AcademiaSinica,Taipei,Taiwan,5DepartmentofDevelopmentandCellBiology,Universityof CaliforniaIrvine,Irvine,California,UnitedStatesofAmerica,6DepartmentofBiologicalChemistry,UniversityofCaliforniaIrvine,Irvine,California,UnitedStatesofAmerica Abstract Heterogeneityofcancerstem/progenitorcellsthatgiverisetodifferentformsofcancerhasbeenwelldemonstratedfor leukemia. However, this fundamental concept has yet to be established for solid tumors including breast cancer. In this communication,weanalyzedsolidtumorcancerstemcellmarkersinhumanbreastcancercelllinesandprimaryspecimens using flow cytometry. The stem/progenitor cell properties of different marker expressing-cell populations were further assessedbyinvitrosoftagarcolonyformationassayandtheabilitytoformtumorsinNOD/SCIDmice.Wefoundthatthe expressionofstemcellmarkersvariedgreatlyamongbreastcancercelllines.InMDA-MB-231cells,PROCRandESA,instead + ofthewidelyusedbreastcancerstemcellmarkersCD44 /CD24-/lowandALDH,couldbeusedtohighlyenrichcancerstem/ progenitorcellpopulationswhichexhibitedtheabilitytoselfrenewanddivideasymmetrically.Furthermore,thePROCR+/ + ESA cells expressed epithelial-mesenchymal transition markers. PROCR could also be used to enrich cells with colony formingabilityfromMB-361cells.Moreover,consistentwiththemarkerprofilingusingcelllines,theexpressionofstemcell markersdifferedgreatlyamongprimarytumors.Therewasanassociationbetweenmetastasisstatusandahighprevalence ofcertainmarkersincludingCD44